Translocation and dissemination of commensal bacteria in post-stroke infection
Translocation and dissemination of commensal bacteria in post-stroke infection
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DOI：
10.1038/nm.4194
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发表时间：
2016-11-01
期刊：
影响因子：
82.9
通讯作者：
Wong, Connie H. Y.
中科院分区：
文献类型：
--
作者：
Stanley, Dragana;Mason, Linda J.;Wong, Connie H. Y.
细菌感染在中风患者中非常普遍。尽管微吸入在中风后肺炎中有潜在的贡献，但我们发现，在中风后发生感染的患者中检测到的大多数微生物都是常见的共生细菌，通常驻留在肠道中。在缺血性中风的小鼠模型中，只有在无特定病原体的设施中出生和长大的小鼠才能观察到中风后感染；在无菌设施中出生和长大的小鼠中没有观察到这一点。利用高通量16S rRNA基因扩增序列测定和生物信息学分析，我们提供的证据表明，形成中风后小鼠肺内微生物群落的细菌确实来自宿主小肠。此外，中风引起的肠道屏障通透性和功能障碍先于口服接种细菌传播到周围组织。这项研究确定了一种新的途径，在这种途径中，中风促进了源于宿主肠道微生物区系的选择性菌株的移位和传播。
Bacterial infection is highly prevalent in patients who have had a stroke. Despite the potential contribution of micro-aspiration in post-stroke pneumonia, we found that the majority of the microorganisms detected in the patients who developed infections after having a stroke were common commensal bacteria that normally reside in the intestinal tracts. In a mouse model of ischemic stroke, post-stroke infection was only observed in mice that were born and raised in specific-pathogen-free facilities; this was not seen in mice that were born and raised in germ-free facilities. Using high-throughput 16S rRNA gene amplicon sequencing and bioinformatics analyses, we provide evidence demonstrating that the source of the bacteria forming the microbial community in the lungs of post-stroke mice was indeed the host small intestine. Additionally, stroke-induced gut barrier permeability and dysfunction preceded the dissemination of orally inoculated bacteria to peripheral tissues. This study identifies a novel pathway in which stroke promotes the translocation and dissemination of selective strains of bacteria that originated from the host gut microbiota.