Humoral immune response in breeding hens and protective immunity provided by administration of purified Salmonella Gallinarum porins.
Humoral immune response in breeding hens and protective immunity provided by administration of purified Salmonella Gallinarum porins.
复制标题
DOI：
10.3382/ps.2009-00448
复制
发表时间：
2010-03
期刊：
影响因子：
4.4
通讯作者：
Gabriela Gómez-Verduzco;G. Téllez;A. L. Quintana;Armando Isibasi;Vianney Ortiz-Navarrete
中科院分区：
文献类型：
--
作者：
Gabriela Gómez-Verduzco;G. Téllez;A. L. Quintana;Armando Isibasi;Vianney Ortiz-Navarrete
本研究旨在评估种鸡的体液免疫反应在后代中提供保护性母体抗体的能力。从鸡沙门氏菌强毒株中分离到一种高纯度的外膜蛋白，其大小为34 kDa。该蛋白与伤寒沙门氏菌孔蛋白发生交叉反应，因此我们认为该外膜蛋白为鸡伤寒沙门氏菌孔蛋白。为评价肉鸡对沙门氏菌的被动免疫效果，将200只肉鸡随机分为3组，分别用10微克、30微克和不含沙门氏菌的PBS进行免疫。肉鸡种鸡血清和受精蛋中均检出抗鸡沙门氏菌孔蛋白抗体(P<；0.05)。因此，与注射PBS的对照母鸡相比，来自免疫肉鸡种鸡的鸡在20至500最大致死量沙门氏菌(P<；0.001)的攻击下获得了53%至70%的保护。这些结果表明，鸡沙门氏菌和其他沙门氏菌一样，参与了被动保护性免疫的诱导，体液免疫应答可能是建立这种保护性免疫的机制之一。
The current studies were undertaken to assess the ability of humoral immune response in breeding hens to provide protective maternal antibody in the progeny. A highly purified outer membrane protein, 34 kDa, was isolated from a virulent strain of Salmonella Gallinarum. Cross-reactivity was observed between this protein and Salmonella Typhi porins; thus we consider this outer membrane protein as a Salmonella Gallinarum porin. To evaluate passive immunity against Salmonella Gallinarum, 200 broiler breeder hens were immunized with either 10 microg of Salmonella Gallinarum porins, 30 microg of Salmonella Gallinarum porins, or PBS without porins as a control group. Anti-Salmonella Gallinarum porin antibodies were detected in broiler breeder serum and in fertile eggs (P < 0.05). Consequently, chickens from immunized broiler breeder hens were protected between 53 to 70% against challenges of 20 to 500 half-maximal lethal dose of Salmonella Gallinarum (P < 0.001) when compared with control hens that were injected with PBS. These results suggest that Salmonella Gallinarum porins, as those of other Salmonella species, participate in the induction of the passive protective immunity, and the humoral immune response may be one of the mechanisms involved in the establishment of this protection.