A conserved activation element in BMP signaling during Drosophila development
A conserved activation element in BMP signaling during Drosophila development
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DOI：
10.1038/nsmb.1715
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发表时间：
2010-01-01
影响因子：
16.8
通讯作者：
Affolter, Markus
中科院分区：
文献类型：
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作者：
Weiss, Alexander;Charbonnier, Enrica;Affolter, Markus
转化生长因子β（TGF-β）家族成员十肢麻痹（Dpp）是果蝇发育中模式和生长的关键调节因子。以前的研究已经确定了一个称为沉默元件（SE）的短DNA基序，它招募了一个三聚体Smad复合物和阻遏物Schnurri，以下调Dpp信号传导后的靶增强子。我们现在已经分离出了dad基因的最小增强子，并发现了一个我们称之为激活元件（AE）的短基序。AE与SE相似，并通过保守机制募集Smad蛋白。然而，AE和SE在重要的核苷酸位置不同。因此，AE不会招募Schnurri，而是通过竞争性DNA结合整合默认阻遏物Brinker的阻遏输入和Smad信号转导器Mothers针对Dpp（Mad）和Medea的激活输入。AE允许鉴定迄今未知的直接Dpp靶标，并且在脊椎动物中功能保守。
The transforming growth factor beta (TGF-beta) family member Decapentaplegic (Dpp) is a key regulator of patterning and growth in Drosophila development. Previous studies have identified a short DNA motif called the silencer element (SE), which recruits a trimeric Smad complex and the repressor Schnurri to downregulate target enhancers upon Dpp signaling. We have now isolated the minimal enhancer of the dad gene and discovered a short motif we termed the activating element (AE). The AE is similar to the SE and recruits the Smad proteins via a conserved mechanism. However, the AE and SE differ at important nucleotide positions. As a consequence, the AE does not recruit Schnurri but rather integrates repressive input by the default repressor Brinker and activating input by the Smad signal transducers Mothers against Dpp (Mad) and Medea via competitive DNA binding. The AE allows the identification of hitherto unknown direct Dpp targets and is functionally conserved in vertebrates.