INDICATOR TECHNIQUE FOR ANTIMETABOLIC TOXIN PRODUCTION BY PHYTOPATHOGENIC SPECIES OF PSEUDOMONAS
INDICATOR TECHNIQUE FOR ANTIMETABOLIC TOXIN PRODUCTION BY PHYTOPATHOGENIC SPECIES OF PSEUDOMONAS
复制标题
DOI：
10.1128/aem.39.1.25-29.1980
复制
发表时间：
1980-01-01
影响因子：
4.4
通讯作者：
GASSON, MJ
中科院分区：
文献类型：
--
作者：
GASSON, MJ
本文建立了一种简单的细菌学技术，即在大肠杆菌草坪上产生抑菌圈，以检测植物病原假单胞菌产生抗代谢毒素的能力。大肠杆菌抑制作用优于植物毒素诱导的植物组织失绿作用。利用这种技术很容易鉴定出不产生抗代谢物的烟草假单胞菌和菜豆假单胞菌的衍生物。质粒DNA在P.结果表明，质粒参与了烟草毒素的产生，但没有发现质粒参与烟草毒素产生的物理证据。对菜豆属也进行了研究。将该方法推广应用于P. maculicola、P.冠状病毒还产生抗代谢物。
A simple bacteriological technique involving inhibition zone production on a lawn ofEscherichia coliwas developed to detect antimetabolite toxin production by phytopathogenic species ofPseudomonas.It was established that the mechanism ofE. coliinhibition paralleled that of phytotoxin-induced chlorosis of plant tissue. Derivatives ofPseudomonas tabaciandPseudomonas phaseolicolawhich did not produce antimetabolite were readily identified by use of this technique. The presence of plasmid DNA inP. tabacistrains was demonstrated, but no physical evidence for plasmid involvement in tabtoxin production was found. The role of antimetabolite production in the pathogenicity ofP. phaseolicolawas also investigated. The method was extended to show that strains ofP. maculicola, P. syringae, andP. coronofaciensalso produce antimetabolites.