Comparison of components of the testis interstitium with testosterone secretion in hamster, rat, and guinea pig testes perfused in vitro.
Comparison of components of the testis interstitium with testosterone secretion in hamster, rat, and guinea pig testes perfused in vitro.
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DOI：
10.1002/aja.1001810103
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
S. Mendis‐Handagama;B. Zirkin;L. Ewing
中科院分区：
文献类型：
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作者：
S. Mendis‐Handagama;B. Zirkin;L. Ewing
用形态计量学技术对金黄地鼠、大鼠和豚鼠的睾丸成分和间质细胞器进行了定量研究。测定了这三种动物体外灌流的睾丸对黄体生成素(L H)(100 ng/ml)刺激的每克睾丸和每个间质细胞的睾酮分泌量。测量了结构之间以及结构与睾酮分泌之间的大量相关性，以提供睾丸内对间质细胞功能控制的结构证据。在三个物种中，转基因睾丸和每个间质细胞的睾酮分泌显著不同：豚鼠最高，大鼠居中，仓鼠最低。生精小管体积与睾丸间质、间质、间质间质和淋巴管体积呈负相关，睾丸间质、间质间质和淋巴管体积与睾酮分泌呈正相关。没有观察到血管体积、细长的纺锤形细胞或每克睾丸的巨噬细胞与睾酮分泌之间的相关性。间质细胞的平均体积与每个间质细胞的光滑内质网(SER)和过氧化物体的体积和表面积呈正相关，溶酶体的体积和线粒体内膜表面积与睾酮的分泌呈负相关。粗面内质网(RER)、高尔基体、脂质的体积和表面积与睾丸间质细胞分泌睾酮的体积、核糖体、胞质基质和核的体积之间无相关性。这些结果表明，间质细胞的大小比间质细胞的数量更重要地解释了这三个物种之间睾酮分泌能力的差异，并且间质细胞平均体积的增加与SER和过氧化体的体积和表面积的增加特别相关。一个重要的悬而未决的问题是，过氧化体在间质细胞类固醇合成中的作用是什么。
Components of the testis and cytoplasmic organelles in Leydig cells were quantified with morphometric techniques in hamster, rat, and guinea pig. Testosterone secretory capacity per gram of testis and per Leydig cell in response to luteinizing hormone (LH) (100 ng/ml) stimulation was determined in these three species from testes perfused in vitro. Numerous correlations were measured among structures, and between structures and testosterone secretion, to provide structural evidence of intratesticular control of Leydig cell function. Testosterone secretion per gm testis and per Leydig cell was significantly different in the three species: highest in the guinea pig, intermediate in the rat, and lowest in the hamster. The volume of seminiferous tubules per gm testis was negatively correlated, and the volumes of interstitium, Leydig cells, and lymphatic space per gm testis were positively correlated with testosterone secretion. No correlations were observed between volumes of blood vessels, elongated spindleshaped cells, or macrophages per gm testes and testosterone secretion. The average volume of a Leydig cell and the volume and surface area of smooth endoplasmic reticulum (SER) and peroxisomes per Leydig cell were positively correlated, and the volume of lysosomes and surface area of inner mitochondrial membrane per Leydig cell were negatively correlated with testosterone secretion. No correlations were observed between volume and surface area of rough endoplasmic reticulum (RER), Golgi apparatus, and lipid, and volume of ribosomes, cytoplasmic matrix, and the nucleus with testosterone secretion per Leydig cell. These results suggest that Leydig cell size is more important than number of Leydig cells in explaining the difference in testosterone-secreting capacity among the three species, and that this increase in average volume of a Leydig cell is associated specifically with increased volume and surface area of SER and peroxisomes. An important unresolved question is what is the role of peroxisomes in Leydig cell steroidogenesis.