The coiled tail of the round-headed spermatozoa appears during epididymal passage in GOPC-deficient mice.
The coiled tail of the round-headed spermatozoa appears during epididymal passage in GOPC-deficient mice.
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DOI：
10.1679/aohc.67.361
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发表时间：
2004-11
影响因子：
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通讯作者：
F. Suzuki-Toyota;C. Ito;Y. Toyama;M. Maekawa;R. Yao;T. Noda;K. Toshimori
中科院分区：
文献类型：
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作者：
F. Suzuki-Toyota;C. Ito;Y. Toyama;M. Maekawa;R. Yao;T. Noda;K. Toshimori
缺失编码GOPC（含有高尔基体相关PDZ和卷曲螺旋基序的蛋白质）的基因的雄性小鼠是不育的，显示出具有卷曲尾的球形精子症（Yao等人，2002年）。我们证实了如何和在哪里尾部异常精子细胞和附睾精子的光镜和电子显微镜。在精子形成过程中，尾部形成正常，但在后环发现缺陷。此后，在附睾通过过程中，精子尾部明显变形。附睾头近端的尾部保持正常、平直，但在附睾尾多盘绕在核周围。推测卷曲是由于缺乏后环而导致细胞质液滴迁移。螺旋尾连接段常脱位或与植入窝分离。许多线粒体从外致密纤维（ODF）中分离出来，形成分层的线粒体鞘。由于这一点，中段的远端部分变得裸露的线粒体鞘。裸露的ODF通常弯曲且无组织。尾部畸形归因于以下结构之间的弱粘附或不完全粘附：1）后环处的质膜和核被膜，2）连接片和植入窝，以及3）线粒体和ODF。这些缺陷分别导致卷曲的尾部、尾部从着床窝脱位以及分层的线粒体鞘伴随中段裸露的ODF。因此，后环在防止卷曲尾形成方面具有重要意义。GOPC缺陷的精子不仅为头部畸形，而且为尾部畸形提供了一个有价值的模型。
Male mice deleting the gene encoding GOPC (Golgi-associated PDZ- and coiled-coil motif-containing protein) are infertile, showing globozoospermia with a coiled tail (Yao et al., 2002). We confirmed how and where tail anomalies were produced in spermatids and epididymal spermatozoa by light and electron microscopy. During spermiogenesis, tail formation occurred normally, but a defect was found at the posterior ring. Thereafter, remarkable sperm tail deformations were induced during epididymal passage. In the proximal caput epidiymidis, the tails remained normal and straight, but most of them coiled around the nucleus in the cauda epididymidis. Coiling is presumed to occur with the migration of the cytoplasmic droplet by the absence of the posterior ring. The connecting piece of the coiled tail was often dislocated or separated from the implantation fossa. Many mitochondria were separated from the outer dense fibers (ODFs) and formed a stratified mitochondrial sheath. Due to this, the distal part of the midpiece became bared of the mitochondrial sheath. The bared ODFs were often bent and disorganized. Tail deformities are attributed to weak or incomplete adhesion between the following structures: 1) plasma membrane and nuclear envelope at the posterior ring, 2) connecting piece and implantation fossa, and 3) mitochondria and ODFs. These defects result in a coiled tail, tail dislocation from the implantation fossa, and the stratified mitochondrial sheath accompanying bared ODFs in the midpiece, respectively. Thus the posterior ring is significant in preventing coiled tail formation. The GOPC-deficient spermatozoa provide a valuable model not only for head but also for tail anomalies.