Isolation and identification of antioxidative peptides from hydrolysate of threadfin bream surimi processing byproduct
Isolation and identification of antioxidative peptides from hydrolysate of threadfin bream surimi processing byproduct
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DOI：
10.1016/j.jff.2013.07.009
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发表时间：
2013-10
影响因子：
5.6
通讯作者：
Chompoonuch Wiriyaphan;B. Chitsomboon;S. Roytrakul;Jirawat Yongsawadigul
中科院分区：
文献类型：
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作者：
Chompoonuch Wiriyaphan;B. Chitsomboon;S. Roytrakul;Jirawat Yongsawadigul
本研究的目的是确定从鲷鱼副产物中提取的部分纯化肽的抗氧化活性和可能的作用方式。用virgibacillussp对脱骨过程中得到的骨架、骨和皮肤（FBS）副产物进行了水解。SK33蛋白酶，用阴离子交换和大小排斥色谱法分离。得到B1、B2、B3三个部分，采用LC-MS/MS测定三个部分中多肽的氨基酸序列。分数B2和B3比分数B1含有更多的Trp、Met、Cys和Tyr残基。B3部位表现出较高的ABTS自由基清除能力和铁还原抗氧化能力（FRAP），而B2和B3部位肽的主要作用方式是金属螯合和羟基自由基清除能力。此外，当HepG2细胞被H2O2处理时，部分B1和从部分B3汇集的新肽中选择的合成肽FLGSFLYEYSR具有清除细胞自由基的作用。
The objective of this study was to determine the antioxidant activity and possible mode of action of partially purified peptides derived from threadfin bream surimi byproduct. The frame, bone and skin (FBS) byproduct, which was obtained from a deboning process, was hydrolyzed byVirgibacillussp. SK33 proteinase and fractionated using anion exchange and size exclusion chromatography. Three fractions, i.e., B1, B2 and B3, were obtained, and the amino acid sequences of the peptides in all 3 fractions were determined using LC-MS/MS. Fractions B2 and B3 contained higher amounts of Trp, Met, Cys and Tyr residues than fraction B1. Fraction B3 exhibited high ABTS radical scavenging activity and ferric reducing antioxidant power (FRAP), while metal chelation and hydroxyl radical scavenging ability were principle modes of action of peptides in fraction B2 and B3. In addition, fraction B1 and a synthetic peptide selected from the pooledde novopeptides of fraction B3, FLGSFLYEYSR, had a cellular radical scavenging effect when HepG2 cells were treated with H2O2.