"Click" chemistry mediated construction of cationic curdlan nanocarriers for efficient gene delivery
"Click" chemistry mediated construction of cationic curdlan nanocarriers for efficient gene delivery
复制标题
“点击”化学介导的阳离子凝胶多糖纳米载体的构建用于有效的基因递送
DOI：
10.1016/j.carbpol.2017.01.055
复制
发表时间：
2017-05-01
影响因子：
11.2
通讯作者：
Baigude, Huricha
中科院分区：
文献类型：
--
作者：
Han, Jingfen;Wang, Xia;Baigude, Huricha
利用“点击化学”技术成功地将阳离子基团定量、选择性地引入到可得然胶的各个葡萄糖单元的C6位上。所得的阳离子可德兰-咪唑-赖氨酸聚合物（Cur-6- 100 Lys）表现出优异的水溶性。通过红外光谱和核磁共振谱对配合物的结构进行了表征，GPC、DLS和SEM对配合物的结构进行了表征。分别进行了细胞毒性测定、电泳迁移率改变实验和EGFP-pDNA转染实验。结果清楚地表明，Cur-6- 100 Lys纳米载体迅速结合dsDNA，对7901细胞和HeLa细胞的细胞毒性较小，并且能够容易地将EGFP-pDNA转运到HepG 2细胞中。我们的研究表明，Cur-6- 100 Lys可以作为一个多功能的纳米平台，在活细胞中进行有效的基因递送。(C)2017爱思唯尔有限公司版权所有
A cationic group has been quantitatively and selectively introduced into C6 position of each glucose units of Curdlan by "Click Chemistry" successfully. The resulting cationic Curdlan-Imidazole-lysine polymers (Cur-6-100Lys) exhibit excellent water solubility. Structure of the Cur-6-100Lys complexes was verified by FTIR and NMR spectroscopic measurements, and analysis of Cur-6-100Lys by GPC, DLS and SEM revealed that they have stoichiometric, nanosized spheroidal structures. Cytotoxicity measurement, electrophoretic mobility shift assay and EGFP-pDNA transfection have been carried out respectively. The results clearly show that Cur-6-100Lys nanocarriers have bound to dsDNA promptly, are less cytotoxic to both 7901 cells and HeLa cells, and are readily able to transport EGFP-pDNA into HepG2 cells. Our studies indicated that Cur-6-100Lys can potentially be used as a versatile nano platform for efficient gene delivery in living cells. (C) 2017 Elsevier Ltd. All rights reserved.