Transcription factor osterix modified bone marrow mesenchymal stem cells enhance callus formation during distraction osteogenesis
Transcription factor osterix modified bone marrow mesenchymal stem cells enhance callus formation during distraction osteogenesis
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DOI：
10.1016/j.tripleo.2010.05.012
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发表时间：
2011-04-01
期刊：
影响因子：
--
通讯作者：
Li, Shu
中科院分区：
文献类型：
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作者：
Lai, Qing-Guo;Yuan, Kui-Feng;Li, Shu
本研究采用兔下颌骨延长模型，观察转染或未转染Osterix（OSX）基因的骨髓间充质干细胞（BMMSCs）局部移植对牵张区骨再生的影响。新西兰白色兔54只，随机分为A、B、C 3组，每组18只。在牵张结束时，A、B、C组分别将自体骨髓基质干细胞和生理盐水注入牵张间隙。每组9只动物在完成牵张后2周和6周人道处死。收集并处理牵引的下颌骨进行放射学、组织学和免疫组织化学检查。A组和B组骨痂成骨良好，A组骨密度、新生骨小梁厚度和新生骨面积最大。与A组和B组相比，C组动物在牵张骨痂中显示出较差的骨形成。骨涎蛋白（BSP）在各组牵张骨痂中均呈阳性表达，但A组BSP表达明显强于B、C组。这项研究的结果表明，BMMSCs移植可以促进骨形成在DO; OSX介导的离体基因治疗是更有效的骨沉积和骨痂形成在牵张成骨。(Oral口腔外科医学口腔病理学口腔放射学Endod 2011;111：412-419）
This study was designed to investigate the effects of local delivery of bone marrow mesenchymal stem cells (BMMSCs) with or without osterix (OSX) gene transfected on bone regeneration in the distracted zone using a rabbit model of mandibular lengthening. Fifty-four New Zealand white rabbits underwent osteodistraction of the left mandible and were then randomly divided into group A, group B, and group C (n = 18 for each group). At the end of distraction BMMSCs transfected with OSX, autologous BMMSCs and physiological saline were injected into the distraction gaps in groups A, B, and C, respectively. Nine animals from each group were humanely killed at 2 and 6 weeks after completion of distraction. The distracted mandibles were harvested and processed for radiographic, histological, and immunohistochemical examination. Excellent bone formation in the distracted callus was observed in group A and group B; the former showed better bone formation and highest bone mineral density (BMD), thickness of new trabeculae (TNT, mm) and volumes of the newly formed bone area (NBV) in the distraction zones. Group C animals showed poor bone formation in the distracted callus when compared with groups A and B. Positive immunostaining of bone sialoprotein (BSP) was observed in the distracted callus in all groups; however, BSP expression was much stronger in group A than in groups B and C. The results of this study suggest transplantation of BMMSCs can promote bone formation in DO; OSX-mediated ex vivo gene therapy was more effective during bone deposition and callus formation in distraction osteogenesis. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011;111:412-419)