Differentiating zones at periodontal ligament-bone and periodontal ligament-cementum entheses.
Differentiating zones at periodontal ligament-bone and periodontal ligament-cementum entheses.
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DOI：
10.1111/jre.12281
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发表时间：
2015-12
影响因子：
3.5
通讯作者：
Ho SP
中科院分区：
文献类型：
--
作者：
Lee JH;Pryce BA;Schweitzer R;Ryder MI;Ho SP
骨-牙周膜（PDL）-牙骨质复合体的结构和功能完整性源于软组织（PDL）和硬组织（骨、牙骨质）之间的承载附着位点（附着点）。这些附着位点负责维持骨-PDL-牙骨质复合体的生物力学功能。目的是研究发育中和功能活跃的附着点中关键生物分子时空表达的变化。对 3 周和 3 个月大的 scleraxis (scx)-GFP 转基因小鼠的半下颌骨进行 CD146、CD31、NG2、osterix (OSX) 和骨唾液蛋白 (BSP) 的多标记技术。通过识别胶原原纤维、PDL 成纤维细胞和 PDL 细胞骨架的方向性来评估 PDL 内的主导拉伸区域。在 PDL 骨附着处鉴定出与 CD31+ 脉管系统相邻的 CD146+ 细胞。 3周龄组NG2+细胞位于冠状骨-PDL和根尖牙骨质-PDL附着点，但3个月时，NG2在根尖区和牙槽嵴附着点呈阳性。 NG2 和 OSX 共定位于 PDL 骨和 PDL 牙骨质附着点的类骨质和类牙骨质区域。 BSP 在 3 周龄小鼠的顶端区域很明显。除3周的顶端区域外，胶原纤维、成纤维细胞及其细胞骨架的方向性重叠。牙周膜邻近附着位点区域的生物分子共定位对于在承载骨-PDL-牙齿纤维关节处形成前牙骨质和类骨界面可能至关重要。发育和功能产生的生物物理线索可以调节干细胞的招募和分化，潜在地从血管起源向 PDL 骨和 PDL 牙骨质附着点处的成骨细胞和成牙骨质细胞谱系。研究导致功能附着位点细胞分化的生物物理和生化刺激的耦合效应对于制定再生策略以实现牙周复合体的功能重建至关重要。
The structural and functional integrity of bone-periodontal ligament (PDL)-cementum complex stems from the load-bearing attachment sites (entheses) between soft (PDL) and hard (bone, cementum) tissues. These attachment sites are responsible for maintenance of a bone-PDL-cementum complex biomechanical function. The objective was to investigate changes in spatiotemporal expression of key biomolecules in developing and functionally active entheses. Multilabeling technique was performed on hemimandibles of 3 week and 3 month-old scleraxis (scx)-GFP transgenic mice for CD146, CD31, NG2, osterix (OSX), and bone sialoprotein (BSP). Regions of dominant stretch within the PDL were evaluated by identifying directionality of collagen fibrils, PDL fibroblasts, and PDL cell cytoskeleton. CD146+ cells adjacent to CD31+ vasculature were identified at PDL-bone enthesis. NG2+ cells were located at coronal bone-PDL and apical cementum-PDL entheses in the 3 weeks-old group, but at 3 months, NG2 was positive at the entheses of the apical region and alveolar crest. NG2 and OSX were colocalized at the osteoid and cementoid regions of the PDL-bone and PDL-cementum entheses. BSP was prominent at the apical region of 3 week old mice. The directionality of collagen fibers, fibroblasts, and their cytoskeleton overlapped, except in the apical region of 3 weeks. Colocalization of biomolecules at zones of the periodontal ligament adjacent to attachment sites may be essential for the formation of precementum and osteoid interfaces at a load-bearing bone-PDL-tooth fibrous joint. Biophysical cues resulting from development and function can regulate recruitment and differentiation of stem cells potentially from vascular origin toward osteo- and cemento-blastic lineages at the PDL-bone and PDL-cementum entheses. Investigating the coupled effect of biophysical and biochemical stimuli leading to cell differentiation at the functional attachment sites is critical for developing regeneration strategies to enable functional reconstruction of the periodontal complex.