INSTRUMENT-DEPENDENT FLUOROCHROME SENSITIVITY IN FLOW CYTOMETRIC ANALYSES
INSTRUMENT-DEPENDENT FLUOROCHROME SENSITIVITY IN FLOW CYTOMETRIC ANALYSES
复制标题
DOI：
10.1002/cyto.990220311
复制
发表时间：
1995-09-15
期刊：
影响因子：
--
通讯作者：
COX, DL
中科院分区：
文献类型：
--
作者：
CHANCE, JT;LARSEN, SA;COX, DL
流式细胞术已成为进行关键临床评价（如CD 4计数和非整倍体分析）的首选技术。越来越多的关注已经出现了众多的技术，试剂，和不同的流式细胞仪用于确定这些数据。几项研究已经证明，当使用不同的流式细胞仪分析同一样本时，结果存在显著差异。许多研究者也报告了荧光染料依赖性仪器灵敏度。随着越来越多的程序通过细胞计数分析进行，光散射和荧光限制（其似乎是仪器依赖性的）表明并非所有流式细胞仪都具有相同的能力。尝试使用流式细胞术标准公司（FCSC）生产的异硫氰酸荧光素（FITC）和R-藻红蛋白（R-PE）标记的微球参考标准品，在9种不同的流式细胞仪上计算等效可溶性荧光染料（MESF）值的分子。观察到某些细胞仪分辨这些微球的能力存在显著差异，分辨率降低似乎与仪器型号和荧光染料有关。我们建议，在某些流式细胞仪荧光分辨率降低可能是负责从实验室报告的临床值的显着变化，利用不同的流式细胞仪。(C)1995 Wiley-Liss，Inc.
Flow cytometry has become the preferred technique by which critical clinical evaluations are made such as CD4 counts and aneuploid analyses. Mounting concern has arisen over the numerous techniques, reagents, and different flow cytometers employed to determine these data. Several studies have documented significant differences in results when different flow cytometers are utilized to analyze the same sample. Fluorochrome-dependent instrument sensitivity also has been reported by numerous investigators. As more and more procedures are performed by cytometric analysis, light scatter and fluorescence limitations, which appear to be instrument dependent, demonstrate that not all flow cytometers have the same capabilities. Attempts were made to calculate molecules of equivalent soluble fluorochrome (MESF) values on nine different flow cytometers using fluorescein isothiocyanate (FITC) and R-phycoerythrin (R-PE) labeled microsphere reference standards produced by Flow Cytometry Standards Corporation (FCSC). Dramatic differences were observed in the ability of some cytometers to resolve these microspheres, The diminished resolution appeared to be instrument model and fluorochrome dependent. We propose that diminished fluorescence resolution in certain flow cytometers could be responsible for significant variability in clinical values reported from laboratories utilizing different flow cytometers. (C) 1995 Wiley-Liss, Inc.