Evaluation of three growth factors for TMJ disc tissue engineering
Evaluation of three growth factors for TMJ disc tissue engineering
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DOI：
10.1007/s10439-005-1741-y
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发表时间：
2005-03-01
影响因子：
3.8
通讯作者：
Athanasiou, KA
中科院分区：
文献类型：
--
作者：
Detamore, MS;Athanasiou, KA
颞下颌关节（TMJ）是人体中最复杂的关节之一，也是现代医学中最困难的问题之一。组织工程，特别是颞下颌关节盘，已被提出作为一个潜在的突破性治疗策略的颞下颌关节疾病。组织工程的核心是了解生长因子对TMJ椎间盘细胞的影响，据我们所知，这是第一个针对这些细胞的3D生长因子研究。目的是研究高和低浓度的碱性成纤维细胞生长因子（bFGF），胰岛素样生长因子-I（IGF）和转化生长因子-β（1）（TGF-β）对猪颞下颌关节盘细胞的影响。将细胞接种到旋转瓶中的非织造PGA支架（95%孔隙率）上，然后用生长因子培养6周。分析构建体的机械和结构完整性、细胞数量和基质生物合成。与对照相比，所有生长因子均改善了机械和结构完整性。IGF和TGF-β在促进胶原蛋白合成方面最有效，尽管任何组之间的糖胺聚糖合成或细胞数量没有显著差异。在考虑了IGF相对于TGF-β的经济优势后，本研究的结论是在未来的TMJ椎间盘组织工程实验中使用IGF。
Arguably one of the most complex joints in the body, the temporomandibular joint (TMJ) presents one of the most difficult problems in modern medicine. Tissue engineering, for the TMJ disc in particular, has been proposed as a potential breakthrough treatment strategy for TMJ disorders. Central to tissue engineering is understanding growth factor effects on TMJ disc cells, and to the best of our knowledge, this is the first 3D growth factor study for these cells. The purpose was to examine the effects of high and low concentrations of basic fibroblast growth factor (bFGF), insulin-like growth factor-I (IGF), and transforming growth factor-beta(1) (TGF-beta) on porcine TMJ disc cells. Cells were seeded onto non-woven PGA scaffolds (95% porosity) in spinner flasks, then cultured with a growth factor for 6 weeks. Constructs were analyzed for mechanical and structural integrity, cell number, and matrix biosynthesis. All growth factors improved mechanical and structural integrity compared to the control. IGF and TGF-beta were most effective at promoting collagen synthesis, although there were no significant differences in glycosaminoglycan synthesis or cell number between any groups. After considering the economic advantage of IGF over TGF-beta, the conclusion of this study is to use IGF in future TMJ disc tissue engineering experiments.