Immunization against endogenous inhibin increases normal oocyte/embryo production in adult mice
Immunization against endogenous inhibin increases normal oocyte/embryo production in adult mice
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DOI：
10.1385/endo:24:2:115
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发表时间：
2004-07-01
期刊：
影响因子：
3.7
通讯作者：
Taya, K
中科院分区：
文献类型：
--
作者：
Medan, MS;Wang, HB;Taya, K
本研究的目的是通过免疫中和内源性抑制素诱导成年小鼠超数排卵，并研究体内和体外胚胎发育。在4d发情周期的发情后期12：00h，用抑制素抗血清(抑制素-AS；50、100或200)对3mo龄的成年雌性小鼠进行超数排卵。对照组用100只正常山羊血清(对照血清)。每隔6h处死5只小鼠，采集血样进行激素测定。每只雌性小鼠在发情前期的下午与能生育的雄性小鼠交配。在交配后第2天的08.00h收集胚胎，在KSOM溶液中培养。经抑制素-AS处理后，血浆FSH浓度明显升高。此外，与对照组相比，抑制素免疫组的血浆雌二醇-17β和孕酮浓度显著升高。体内正常受精的超排卵母细胞在体外能够正常发育到囊胚。经抑制素-AS处理的小鼠卵母细胞和囊胚数显著高于对照组小鼠。抑制素-AS处理组的受精率和囊胚发育率与对照组相似。这些结果表明，免疫中和内源性抑制素诱导成年小鼠超数排卵，而不需要额外的人绒毛膜促性腺激素(HCG)处理。此外，应用抑制素-AS获得的超排卵母细胞具有正常的胚胎发育能力。
The objectives of the present study were to induce superovulation in adult mice by immunoneutralization of endogenous inhibin and to investigate embryo development in vivo and in vitro. Adult female mice of the ddY strain at 3 mo of age were superovulated with a single ip injection of inhibin antiserum (inhibin-AS; 50, 100, or 200 muL) at 12:00 h on metestrus of the 4-d estrous cycle. The control group was treated with 100 muL normal goat serum (control serum). Five mice were sacrificed every 6 h and blood samples were collected for hormonal assay. Individual female mice were bred in the afternoon of proestrus with individual fertile males. Embryos were collected at 08.00 h on d 2 post-coitus and cultured in KSOM solution. Treatment with inhibin-AS significantly increased the concentrations of plasma FSH. Furthermore, there was a significant elevation in plasma concentrations of estradiol-17beta and progesterone in the inhibin-immunized groups in comparison with controls. The superovulated oocytes that were fertilized normally in vivo were able to develop normally to blastocysts in vitro. The number of oocytes and blastocysts developed in animals treated with inhibin-AS was significantly higher than that for mice treated with control serum. Moreover, the rate of fertilization and the rate of blastocyst development were similar in inhibin-AS treated and control groups. These results indicate that immunoneutralization of endogenous inhibin induces superovulation in adult mice without additional treatment with human chorionic gonadotropin (hCG). In addition, the superovulated oocytes obtained by administration of inhibin-AS have normal embryonic developmental competence.