Gene islands integrated into tRNAGly genes confer genome diversity on a Pseudomonas aeruginosa clone
Gene islands integrated into tRNAGly genes confer genome diversity on a Pseudomonas aeruginosa clone
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DOI：
10.1128/jb.184.23.6665-6680.2002
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发表时间：
2002-12-01
影响因子：
3.2
通讯作者：
Tümmler, B
中科院分区：
文献类型：
--
作者：
Larbig, KD;Christmann, A;Tümmler, B
在铜绿假单胞菌染色体最多样化的马赛克区域之一研究了铜绿假单胞菌的克隆内基因组多样性。对铜绿假单胞菌（P. aeruginosa）优势克隆C株C和菌株SG17M的两个成员UpH基因附近约110 kb的大高变区进行了测序。在这两种菌株中，该区域由111个(菌株C或106个（SG17M）开放阅读框（orf）的单个菌株特异性基因岛和7 kb的9个orf的克隆C特异性序列组成。这些基因岛被整合到保守的tRNA（Gly）基因中，具有两部分结构。与tRNA基因相邻的第一部分由菌株特异性orf组成，这些orf编码代谢功能和转运体，其中大多数在其他真细菌中具有已知功能的同源物，如造血细胞、细胞色素c生物合成或抗汞性。第二部分主要由未知函数的orf组成。其中47个orf具有互同源性，氨基酸序列同源性为35% ~ 88%，在两个基因岛中排列顺序相同。我们假设这种新型的基因岛来源于可移动的元素，这些元素在整合后赋予受体特定的代谢特性，从而可能赋予其在特定栖息地的选择优势。
Intraclonal genome diversity of Pseudomonas aeruginosa was studied in one of the most diverse mosaic regions of the P. aeruginosa chromosome. The ca. 110-kb large hypervariable region located near the UpH gene in two members of the predominant P. aeruginosa clone C, strain C and strain SG17M, was sequenced. In both strains the region consists of an individual strain-specific gene island of 111 (strain C or 106 (SG17M) open reading frames (ORFs) and of a 7-kb stretch of clone C-specific sequence of 9 ORFs. The gene islands are integrated into conserved tRNA(Gly) genes and have a bipartite structure. The first part adjacent to the tRNA gene consists of strain-specific ORFs encoding metabolic functions and transporters, the majority of which have homologs of known function in other eubacteria, such as hemophores, cytochrome c biosynthesis, or mercury resistance. The second part is made up mostly of ORFs of yet-unknown function. Forty-seven of these ORFs are mutual homologs with a pairwise amino acid sequence identity of 35 to 88% and are arranged in the same order in the two gene islands. We hypothesize that this novel type of gene island derives from mobile elements which, upon integration, endow the recipient with strain-specific metabolic properties, thus possibly conferring on it a selective advantage in its specific habitat.