Human hybridomas from patients with malignant disease.
Human hybridomas from patients with malignant disease.
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DOI：
10.1038/bjc.1983.16
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发表时间：
1983-01
影响因子：
8.8
通讯作者：
Watson, J
中科院分区：
文献类型：
--
作者：
Sikora, K;Alderson, T;Ellis, J;Phillips, J;Watson, J
收集180例多种恶性疾病患者的淋巴细胞，与人骨髓瘤细胞系LON-LICR-HMy2/CAM1融合。共获得162株杂交瘤细胞。融合产物表面仅有B淋巴细胞标志物。流式细胞仪分析显示杂交瘤细胞DNA含量稳定增加。一些杂交瘤上清液中含有新的Ig链。在12种上清液中发现了抗肿瘤结合活性。
Lymphocytes from 180 patients with a variety of malignant diseases were collected and fused with a human myeloma-derived line, LON-LICR-HMy2/CAM1. A total of 162 hybridomas was obtained. Only B lymphocyte markers were found on the surface of the fusion products. Flow cytometric analysis revealed a stably increased DNA content in the hybridoma cells. Some hybridoma supernatants were found to contain new Ig chains. Anti-tumour binding activity was found in 12 supernatants.