Characterization of the S-RNase genomic DNA allele sequence in Prunus speciosa and P. pseudocerasus
Characterization of the S-RNase genomic DNA allele sequence in Prunus speciosa and P. pseudocerasus
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大花李和假樱桃中 S-RNase 基因组 DNA 等位基因序列的表征
DOI：
10.1016/j.scienta.2012.06.041
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发表时间：
2012-09-06
影响因子：
4.3
通讯作者：
Zhang, Shao-Ling
中科院分区：
文献类型：
--
作者：
Gu, Chao;Wu, Jun;Zhang, Shao-Ling
本研究利用PCR技术从2份樱桃李和2份大果李的基因组DNA中分离到8个S-RNase等位基因。氨基酸序列分析显示五个新的和三个公布的S-等位基因。这些S-RNases具有与其它表现配子体自交不亲和的李属物种相同的典型结构特征。大果李4个S-RNase等位基因的氨基酸同源性为60.8 ~ 75.6%，樱桃李4个S-RNase等位基因的氨基酸同源性为73 ~ 81.4%。第一内含子的大小范围为197 - 341 bp，第二内含子的大小范围为81 - 1182 bp。序列分析表明，与其他李属物种相比，推导的氨基酸同一性往往高于种内同一性。Pspe-S-1与Pd-S-28、Pspe-S-31与Pm-S-6、Pspe-S-51、Pa-S-29与Pweb-S-7、Pps-S-13、Psim-S-4与Ps-S-B之间的同源性较高，表明S-RNase等位基因在李属物种分化之前就已进化。有趣的是，两个S-RNase等位基因之间的第一和第二内含子的相似性也很高，第一内含子之间的相似性范围为83.63%至100%，第二内含子之间的相似性范围为46.13%至100%。这些信息并不能增加我们对李属物种S等位基因的了解，但可用于果树的分子育种，以避免杂交不亲和性。(C)2012 Elsevier B. V.保留所有权利。
In this study, eight S-RNase alleles were isolated from two Prunus pseudocerasus and two Prunus speciosa accessions by PCR amplification from genomic DNA. Analysis of the amino acid sequences revealed five novel and three published S-alleles. These S-RNases share typical structural features with S-RNases from other Prunus species exhibiting gametophytic self-incompatibility. The deduced amino acid identities ranged from 60.8 to 75.6% among four S-RNase alleles in Prunus speciosa and ranged from 73 to 81.4% among four S-RNase alleles in Prunus pseudocerasus. The size of the first introns ranged from 197 to 341 bp, and the size of second introns ranged from 81 to 1182 bp. Sequence analysis demonstrated that the deduced amino acid identities, by comparison with other Prunus species, were often higher than those of intraspecific identities. Moreover, exceptionally high identities were found between Pspe-S-1 and Pd-S-28; between Pspe-S-31 and Pm-S-6; among Pspe-S-51, Pa-S-29 and Pweb-S-7; and among Pps-S-13, Psim-S-4 and Ps-S-b, indicating that the S-RNase alleles evolved before Prunus species divergence. Interestingly, the similarities of the first and second introns were also high between the two S-RNase alleles, which range from 83.63 to 100% among the first introns and from 46.13 to 100% among the second introns. These information could not increase our knowledge on the S-alleles of Prunus species, but is available for molecular breeding of fruit trees, to avoid cross-incompatibility. (C) 2012 Elsevier B.V. All rights reserved.