Bicarbonate is not the ultimate answer to the biocompatibility problems of CAPD solutions: a cytotoxicity test of CAPD solutions and effluents.
Bicarbonate is not the ultimate answer to the biocompatibility problems of CAPD solutions: a cytotoxicity test of CAPD solutions and effluents.
复制标题
碳酸氢盐并不是 CAPD 溶液生物相容性问题的最终答案：CAPD 溶液和流出物的细胞毒性测试。
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
P. Wang
中科院分区：
文献类型：
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作者：
H. Schambye;F. B. Pedersen;P. Wang
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在暴露于CAPD溶液和透析第一个小时期间从使用乳酸盐或碳酸氢盐基CAPD溶液治疗的患者采集的流出液后，检测人多形粒细胞（PMN）的迁移和吞噬作用。与标准细胞培养基中获得的值相比，乳酸盐溶液（Dianeal和Lockolys）的流出物减少了迁移并增强了吞噬作用。在透析期间，两种细胞功能均增加。相比之下，当使用87 b，基于碳酸氢盐的CAPD溶液（pH = 7.4，[HCO 3-]= 29 mM）时，细胞功能仅轻微改变。在前30分钟期间，当暴露于87 b流出物时，细胞以比暴露于乳酸盐流出物时更高的水平运行。观察结果进一步表明，PMN的最佳条件是碳酸氢盐浓度小于20 mM，乳酸盐浓度小于15 mM。
最后
基于乳酸盐和碳酸氢盐的CAPD溶液和透析第一个小时期间收集的流出液均减少了PMN迁移。CAPD溶液的生物相容性可以通过将乳酸盐和碳酸氢盐缓冲系统组合在具有小于20 mM碳酸氢盐和小于15 mM乳酸盐的浓度的溶液中来改善。
UNLABELLED
Human polymorphonuclear granulocytes (PMN) were tested for migration and phagocytosis after exposure to CAPD solutions and effluents sampled during the first hour of dialysis from patients treated with lactate or bicarbonate based CAPD-solutions. The effluents from the lactate based solutions (Dianeal and Lockolys) reduced the migration and enhanced the phagocytosis compared to values obtained in a standard cell culture medium. Both cell functions increased during the dialysis period. In contrast, the cell-function only changed slightly when 87b, a bicarbonate based CAPD-solution (pH = 7.4, [HCO3-) = 29mM), was employed. During the first 30 minutes, the cells performed at a higher level when exposed to the 87b effluent than when exposed to the lactate effluents. The observations further indicated that optimal conditions for PMNs are at a bicarbonate concentration of less than 20 mM and a lactate concentration of less than 15mM.
IN CONCLUSION
PMN migration is reduced by both lactate and bicarbonate based CAPD solutions and effluents collected during the first hour of dialysis. The bio-compatibility of CAPD solutions may be improved by combining the lactate and bicarbonate buffering systems in a solution with a concentration of less than 20 mM of bicarbonate and less than 15 mM of lactate.