IMMUNOCHEMICAL PROOF THAT A NOVEL REARRANGING GENE ENCODES THE T-CELL RECEPTOR-DELTA SUBUNIT
IMMUNOCHEMICAL PROOF THAT A NOVEL REARRANGING GENE ENCODES THE T-CELL RECEPTOR-DELTA SUBUNIT
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DOI：
10.1126/science.3672118
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发表时间：
1987-10-30
期刊：
影响因子：
56.9
通讯作者：
BRENNER, MB
中科院分区：
文献类型：
--
作者：
BAND, H;HOCHSTENBACH, F;BRENNER, MB
The T cell receptor (TCR) .delta. protein is expressed as part of a heterodimer with TCR .gamma., in association with the CD3 polypeptides on a subset of functional peripheral blood T lymphocytes, thymocytes, and certain leukemic T cell lines. A monoclonal antibody directed against TCR .delta. was produced that binds specifically to the surface of several TCR .gamma..delta. cell lines and immunoprecipitates the TCR .gamma..delta. as a heterodimer from Triton X-100 detergent lysates and also immunoprecipitates the TCR .delta. subunit alone after chain separation. A candidate human TCR .delta. complementary DNA clone (IDP2 O-240/38), reported in a companion paper, was isolated by the subtractive library approach from a TCR .gamma..delta. cell line. This complementary DNA clone was used to direct the synthesis of a polypeptide that is specifically recognized by the monoclonal antibody to TCR .delta.. This complentary DNA clone thus corresponds to the gene that encodes the TCR .delta. subunit.
