The phyB-dependent induction of HY5 promotes iron uptake by systemically activating FER expression
The phyB-dependent induction of HY5 promotes iron uptake by systemically activating FER expression
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DOI：
10.15252/embr.202051944
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发表时间：
2021-05-20
期刊：
影响因子：
7.7
通讯作者：
Yu, Jingquan
中科院分区：
文献类型：
--
作者：
Guo, Zhixin;Xu, Jin;Yu, Jingquan
铁(Fe)缺乏影响全球农作物生产力和人类健康。然而，光信号在植物铁吸收中的作用尚不清楚。在此，我们发现光诱导番茄(Solanum lycopsicum L.)对铁的吸收。在很大程度上依赖于光敏色素B(PhyB)。光诱导叶和根中细长的下胚轴5(HY5)蛋白的PhyB依赖的积累。HY5从茎到根的运动激活了FER转录因子的表达，导致参与铁吸收的转录本的积累。FER的突变消除了光质引起的铁吸收的变化。在PhyB、hy5和fer突变体中观察到的低铁吸收伴随着较低的光合电子传递速率。夜间红光照射增加了野生型果实中铁的积累，但对PhyB突变体的果实影响不大。综上所述，这些结果表明，铁的吸收受到光的系统调控，这种调控方式依赖于PhyB-HY5-FER。这些发现提供了新的见解，即如何利用操纵光质来改善作物对铁的吸收，从而提高作物的营养质量。
Iron (Fe) deficiency affects global crop productivity and human health. However, the role of light signaling in plant Fe uptake remains uncharacterized. Here, we find that light-induced Fe uptake in tomato (Solanum lycopersicum L.) is largely dependent on phytochrome B (phyB). Light induces the phyB-dependent accumulation of ELONGATED HYPOCOTYL 5 (HY5) protein both in the leaves and roots. HY5 movement from shoots to roots activates the expression of FER transcription factor, leading to the accumulation of transcripts involved in Fe uptake. Mutation in FER abolishes the light quality-induced changes in Fe uptake. The low Fe uptake observed in phyB, hy5, and fer mutants is accompanied by lower photosynthetic electron transport rates. Exposure to red light at night increases Fe accumulation in wild-type fruit but has little effects on fruit of phyB mutants. Taken together, these results demonstrate that Fe uptake is systemically regulated by light in a phyB-HY5-FER-dependent manner. These findings provide new insights how the manipulation of light quality could be used to improve Fe uptake and hence the nutritional quality of crops.