Liposome-encapsulated HIV-1 Gag p24 containing lipid A induces effector CD4+T-cells, memory CD8+T-cells, and pro-inflammatory cytokines
Liposome-encapsulated HIV-1 Gag p24 containing lipid A induces effector CD4+T-cells, memory CD8+T-cells, and pro-inflammatory cytokines
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DOI：
10.1016/j.vaccine.2009.08.105
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发表时间：
2009-11-16
期刊：
影响因子：
5.5
通讯作者：
Rao, Mangala
中科院分区：
文献类型：
--
作者：
Steers, Nicholas J.;Peachman, Kristina K.;Rao, Mangala
脂质体脂质 A 是一种有效的佐剂，用于递送抗原以及诱导细胞和体液免疫。在本研究中，我们证明，在用含有脂质 A [L(p24+ LA)] 的脂质体封装的 HIV-1 Gag p24 进行第三次免疫后，中央记忆 CD8+ T 细胞位于小鼠的脾脏和淋巴结中，而效应记忆 CD8+ T 细胞和效应 CD4+ T 细胞则在 PBMC 中发现。还在脾脏和淋巴结中检测到效应 CD4+ T 细胞。脾淋巴细胞和淋巴结分泌的主要细胞因子是IFN-γ。相反，IL-6 和 IL-10 是 PBMC 产生的主要细胞因子。肽刺激表明观察到的细胞因子反应是T细胞特异性的。结果证明，L(p24 + LA) 免疫后，佐剂脂质体脂质 A 对于诱导 HIV-1 Gag p24 特异性 CD8+ T 细胞、效应 CD4+ T 细胞和具有 Th-1 型特征的细胞因子具有重要意义。由爱思唯尔有限公司出版
Liposomal lipid A is an effective adjuvant for the delivery of antigens and for the induction of both cellular and humoral immunity. In this study, we demonstrate that following the third immunization with HIV-1 Gag p24 encapsulated in liposomes containing lipid A [L(p24+ LA)], central memory CD8+ T-cells were localized in the spleen and lymph nodes of mice while effector memory CD8+ T-cells and effector CD4+ T-cells were found in the PBMC. Effector CD4+ T-cells were also detected in the spleen and lymph nodes. The predominant cytokine secreted from splenic lymphocytes and lymph nodes was IFN-gamma. In contrast, IL-6 and IL-10 were the major cytokines produced from PBMC. The peptide stimulation indicated that the cytokine responses observed were T-cell specific. The results demonstrate the importance of the adjuvant liposomal lipid A for the induction of HIV-1 Gag p24-specific CD8+ T-cells, effector CD4+ T-cells, and cytokines with a Th-1 type profile after immunization with L(p24 + LA). Published by Elsevier Ltd.