Transcriptome analysis of golden pompano (Trachinotus ovatus) liver indicates a potential regulatory target involved in HUFA uptake and deposition
Transcriptome analysis of golden pompano (Trachinotus ovatus) liver indicates a potential regulatory target involved in HUFA uptake and deposition
复制标题
金鲳鱼 (Trachinotus ovatus) 肝脏的转录组分析表明了参与 HUFA 吸收和沉积的潜在调控靶点
DOI：
10.1016/j.cbd.2019.100633
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发表时间：
2020-03-01
影响因子：
3
通讯作者：
Li, Yuan-you
中科院分区：
文献类型：
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作者：
Lei, Cai-xia;Li, Meng-meng;Li, Yuan-you
促进高度不饱和脂肪酸（HUFA）的吸收和沉积可以提高养殖鱼类的营养价值，减少饲料鱼油的添加量。在前期研究中，我们发现卵形鲳鲹肝脏HUFA含量随着饲料HUFA水平的增加而增加。因此，我们研究了共同的基因和途径负责HUFA的吸收和沉积在T。在用1.0%或2.1%HUFA喂养8周后，使用转录组测序技术对卵圆形肝脏进行测序。结果显示，总共有140个和147个基因分别显著上调和下调。2.1%HUFA组中与脂肪乳化相关的5个胆汁酸合成相关基因（CYP7A1、CYP8B1、AKR1D1、SCP 2和ACOT8）表达下调，提示经典途径合成的胆酸盐可能是脂肪乳化过程中的主要胆汁酸形式。脂肪酸转运蛋白（FATP）-6、脂肪酸结合蛋白（FABP）-1、-4和-6随着HUFA沉积而增加，尤其是FATP 6和FABP 4，提示这两个基因可能是HUFA摄取和沉积的重要介质。KEGG分析显示，上述差异基因大多参与过氧化物酶体增殖物激活物受体（peroxisome proliferator activator receptor，PPAR γ）信号通路，且随着HUFA沉积而增加，表明PPAR γ可能通过调控脂肪酸乳化和转运相关基因，成为HUFA摄取和沉积的关键调控因子。本研究以中间代谢中心的肝脏为研究对象，全面了解HUFA在T. ovatus，应进一步调查，以制定潜在的措施，以提高HUFA含量。
Promoting highly unsaturated fatty acid (HUFA) uptake and deposition can improve nutritional value of farmed fish and reduce dietary fish oil addition. Previously, we found that the golden pompano Trachinotus ovatus liver HUFA content increased with the increasing of dietary HUFA. Therefore, we examined the common genes and pathways responsible for HUFA uptake and deposition in T. ovatus liver using transcriptome sequencing technology after feeding with either 1.0% or 2.1% HUFA for 8 weeks. Results showed that a total of 140 and 147 genes were significantly upregulated and downregulated, respectively. Five bile acid synthesis-related genes (CYP7A1, CYP8B1, AKR1D1, SCP2 and ACOT8), which are related to dietary fat emulsification were downregulated in 2.1% HUFA group, implying that the cholate synthesized through the classical pathway might be the main bile acid form in fat emulsification. Moreover, fatty acid transport protein (FATP)-6, fatty acid binding protein (FABP)-1, -4, and -6 increased with HUFA deposition, especially FATP6 and FABP4, suggesting that the two genes may be important mediators involved in HUFA uptake and deposition. KEGG analysis showed that most of the differential genes described above were involved in peroxisome proliferator activator receptor (PPAR) signaling pathway, and PPAR gamma increased with HUFA deposition, indicating that PPAR gamma might be a key regulator of HUFA uptake and deposition by regulating the genes involved in fatty acid emulsification and transport. This study focused on the liver, which is the center of intermediary metabolism, providing a comprehensive understanding of the molecular regulation of HUFA uptake and deposition in T. ovatus, which should be further investigated to develop potential measures to improve HUFA content.