No relationship between 18F-fluorodeoxyglucose positron emission tomography and expression of Glut-1 and -3 and hexokinase I and II in high-grade glioma
No relationship between 18F-fluorodeoxyglucose positron emission tomography and expression of Glut-1 and -3 and hexokinase I and II in high-grade glioma
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DOI：
10.3892/or_00000039
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发表时间：
2008-09-01
期刊：
影响因子：
4.2
通讯作者：
Price, P.
中科院分区：
文献类型：
--
作者：
Charnley, N.;Airley, R.;Price, P.
本研究的目的是比较高级别胶质瘤中葡萄糖代谢与Glut-1和-3以及己糖激酶I（Hex I）和II的表达，使用（18）F-氟脱氧葡萄糖正电子发射断层扫描（[（18）F]FDG-PET）测量。这项回顾性研究涉及27例WHO分类III级和IV级胶质瘤患者，这些患者都是新诊断或复发的肿瘤。患者分别接受动态和静态[（18）F]FDG-PET葡萄糖代谢率（MRGIu）和标准化摄取值（SUV）。获得肿瘤活检组织，并使用免疫组织化学染色Glut-1，-3，Hex I和II的表达。使用斯皮尔曼等级相关检验研究变量之间的关系。结果表明，Glut-1、Glut-3、Hex I和Hex II在不同品种间和品种内的表达量存在差异。MRGlu的平均值为0.2（范围0.09-0.25）μ mol/min/ml，SUV的平均值为4.2（范围3.2-5.2）。用MRGIu或SUV研究的任何蛋白质的肿瘤表达之间没有显著关系（所有p > 0.21）。总之，Glut-1，-3，Hex I或11的免疫组化表达与使用[（18）F]FDG-PET测量的高级别胶质瘤患者的葡萄糖代谢之间缺乏相关性可能是由于组织异质性和高级别肿瘤中坏死的存在。
The purpose of this study was to compare glucose metabolism, measured using (18)F-fluorodeoxyglucose positron emission tomography ([(18)F]FDG-PET), with the expression of Glut-1 and -3 and hexokinase I (Hex I) and II in high-grade glioma. The retrospective study involved 27 patients with WHO classification grade III and IV glioma, with either newly diagnosed or recurrent tumours. Patients underwent dynamic and static [(18)F]FDG-PET to glucose metabolic rate (MRGIu) and standardised uptake value (SUV), respectively. Tumour biopsies were obtained and stained using immunohistochemistry for the expression of Glut-1, -3, Hex I and II. Relationships between variables were studied using Spearman's rank correlation test. Results showed that the expression of Glut-1, Glut-3, Hex I and Hex II varied between and within the turnout samples. The mean of MRGlu was 0.2 (range 0.09-0.25) mu mol/min/ml and that of SUV was 4.2 (range 3.2-5.2). There were no significant relationships among the tumour expression of any of the proteins studied with either MRGIu or SUV (p > 0.21 for all). In conclusion, the lack of relationship between the immunohistochemical expression of Glut-1, -3, Hex I or 11 and glucose metabolism measured using [(18)F]FDG-PET in patients with high-grade glioma may be due to the tissue heterogeneity and presence of necrosis in high-grade tumours.