Human cytomegalovirus in blood of immunocompetent persons during primary infection: Prognostic implications for pregnancy
Human cytomegalovirus in blood of immunocompetent persons during primary infection: Prognostic implications for pregnancy
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DOI：
10.1086/515277
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发表时间：
1998-05-01
影响因子：
6.4
通讯作者：
Gerna, G
中科院分区：
文献类型：
--
作者：
Revello, MG;Zavattoni, M;Gerna, G
通过定量测定 pp65 抗原血症、病毒血症和白细胞 DNA 血症，研究了 52 名原发性 HCMV 感染的免疫功能正常患者（40 名孕妇）的外周血白细胞 (PBL) 中的人巨细胞病毒 (HCMV)。发病后 1、2 和 3 个月检查的 21 名患者中有 12 名（57.1%）检测到 pp65 抗原血症，16 名患者中有 4 名（25%）检测到 pp65 抗原血症，10 名患者中有 0 名检测到 pp65 抗原血症。 19 名患者中的 5 名 (26.3%) 仅在第一个月就检测到了病毒血症。在发病后 1、2 和 3 个月进行检测的患者中，分别有 20 名患者中的 20 名、19 名患者中的 17 名（89.5%）和 19 名患者中的 9 名（47.3%）检测出白细胞 DNA 血症。 15 名患者中有 4 名 (26.6%) 在 4-6 个月时仍呈 DNA 血症阳性，而在 > 6 个月时没有人呈阳性。在 20 名 HCMV 免疫供体或 9 名反复感染的血清阳性受试者的 PBL 中未检测到 HCMV。所有检测的病毒水平都很低，并且与感染的临床病程、宫内传播或结果的严重程度无关。在母体白细胞 DNA 血症存在的情况下进行侵入性操作似乎不会干扰 HCMV 感染的垂直传播。
Human cytomegalovirus (HCMV) was investigated in peripheral blood leukocytes (PBL) of 52 immunocompetent patients (40 pregnant women) with primary HCMV infection by quantitative determination of pp65 antigenemia, viremia, and leukoDNAemia. pp65 antigenemia was detected in 12 (57.1%) of 21, 4 (25%) of 16, and 0 of 10 patients examined 1, 2, and 3 months after onset, respectively. Viremia was detected in 5 (26.3%) of 19 patients during the first month only. LeukoDNAemia was detected in 20 of 20, 17 (89.5%) of 19, and 9 (47.3%) of 19 patients tested 1, 2, and 3 months after onset, respectively. Four (26.6%) of 15 patients were still DNAemia-positive at 4-6 months, whereas none were positive at >6 months. HCMV was not detected in PBL of 20 HCMV-immune donors or of 9 seropositive subjects with recurrent infection. Virus levels were low by all assays and did not correlate with clinical course of infection, intrauterine transmission, or severity of outcome. invasive procedures in the presence of maternal leukoDNAemia did not seem to interfere with vertical transmission of HCMV infection.