Biosurfactant-Stabilized Micropore-Forming GelMA Inks Enable Improved Usability for 3D Printing Applications
Biosurfactant-Stabilized Micropore-Forming GelMA Inks Enable Improved Usability for 3D Printing Applications
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DOI：
10.1007/s40883-022-00250-5
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发表时间：
2022-03
影响因子：
2.6
通讯作者：
Xin-Sheng Qin;Mian Wang;Wanlu Li;Y. S. Zhang
中科院分区：
文献类型：
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作者：
Xin-Sheng Qin;Mian Wang;Wanlu Li;Y. S. Zhang
目的三维(3D)生物打印技术在构建细胞工程化组织模型方面具有巨大的潜力。最近，我们开发的一种新型微孔生物墨水，含有两个不相容的明胶甲基丙烯酰基(GelMA)和聚环氧乙烷(PEO)水相，由于它促进了细胞行为，因此引起了人们的关注。然而，这种初始版本的双水乳液生物油墨在室温下储存时间较长时通常不稳定，从而会出现相分离并失去微孔形成能力。这种相分离可能导致生物打印的操作时间窗口不足，特别是对于需要液相生物墨水的方式，如数字光处理。方法在本研究中，我们报道了一组生物表面活性剂(鼠李糖脂)稳定的形成微孔的GelMA基墨水的开发，目的是显著延长其保质期，增强对3D打印的适用性。结果观察到，使用鼠李糖脂稳定的微孔形成墨水的打印结构，无论是新鲜制备的还是在室温下储存数小时，都呈现出类似的微孔结构。相反，没有加入生物表面活性剂的微孔形成油墨在长期储存后由于明显的相分离而表现出严重的性能下降。结论我们的研究表明，生物表面活性剂掺入增强了形成微孔的GelMA油墨的稳定性，因此在进一步开发双相水性乳液油墨和生物油墨用于未来的3D打印和生物打印应用方面提供了广泛的可能性。三维(3D)生物印刷提供了一系列使技术能够解决再生工程和转化医学问题的技术，通过允许精确控制的、自动化的体积组织结构的制造，这些组织结构在结构和功能上与人体中的对应物相关。用于生物打印的生物材料对于确保适当的组织产生和成熟具有重要意义。我们报道了一种由生物衍生表面活性剂稳定的微孔形成油墨，旨在提高3D打印体系中所产生的多孔结构的稳定性，在组织工程和再生医学中具有潜在的应用价值。
PurposeThree-dimensional (3D) bioprinting offers great potentials in rebuilding tissue mimics through engineering cell-laden constructs. Recently, the unique ability of a new type of micropore-forming bioink developed by us, containing two immiscible aqueous phases of gelatin methacryloyl (GelMA) and poly(ethylene oxide) (PEO), has become attractive since it promotes cellular behaviors. Nevertheless, this initial version of our two-phase aqueous emulsion bioink is generally unstable when experiencing prolonged storage times at room temperature, whereby it will phase-segregate and lose the micropore-forming capacity. This phase-segregation may lead to insufficient operational time window for bioprinting, especially for modalities that require a liquid-phase bioink such as digital light processing.MethodsIn this study, we report the development of a set of biosurfactant (rhamnolipids)-stabilized micropore-forming GelMA-based inks, with the goal of significantly improving their shelf-lives with enhanced applicability towards 3D printing.ResultsIt was observed that the printed constructs using rhamnolipid-stabilized micropore-forming inks, either prepared fresh or stored for hours at room temperature, presented similar microporous structures. In contrast, the micropore-forming inks without biosurfactant-incorporation exhibited severely reduced performances after prolonged storage owing to marked phase-segregation.ConclusionOur study suggests that biosurfactant-incorporation enhanced stability of our micropore-forming GelMA inks and therefore present a wide range of possibilities in further development of two-phase aqueous emulsion inks and bioinks for future 3D printing and bioprinting applications.Lay SummaryThree-dimensional (3D) bioprinting offers a collection of enabling technologies to address regenerative engineering and translational medicine problems, by allowing precisely controlled, automated fabrication of volumetric tissue constructs that are both structurally and functionally relevant to their counterparts in the human body. The biomaterials used for bioprinting are of significant importance to ensure proper tissue-production and maturation. We report a micropore-forming ink that is stabilized by biologically derived surfactant, in an effort to promote the stability of the resulting porous structures in 3D-printed architectures, for potential applications in tissue engineering and regenerative medicine.