Continuous production of structured phospholipids in a packed bed reactor with lipase from Thermomyces lanuginosa
Continuous production of structured phospholipids in a packed bed reactor with lipase from Thermomyces lanuginosa
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使用来自疏棉状嗜热丝孢菌的脂肪酶在填充床反应器中连续生产结构化磷脂
DOI：
10.1007/s11746-005-1061-x
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Xuebing Xu
中科院分区：
文献类型：
--
作者：
A. F. Vikbjerg;Lifeng Peng;H. Mu;Xuebing Xu
研究了在连续固定床酶反应器中脂肪酶催化酸解大豆磷脂和辛酸制备结构磷脂的可能性。酸解反应在溶剂体系和无溶剂体系中进行，以来自Thermomobacteriumlanestine的商业固定化脂肪酶（Lipozyme TL IM）作为催化剂。在填充床反应器中，阐明了脂肪酶催化酸解的不同参数，如溶剂比（溶剂系统），温度，底物比，停留时间，含水量和操作稳定性。在填充床反应器中，水的含量对酸解反应的进行是非常关键的。如果在无溶剂体系下的反应期间没有水加入到底物中，则观察到辛酸的非常低的掺入结合。在溶剂和无溶剂体系中，酰基掺入有利于酰基供体和磷脂之间的高底物比，较长的停留时间，和较高的反应温度。在一定条件下，以正己烷为溶剂，连续操作可获得约30%辛酸的掺入。
The possibilities of producing structured phospholipids between soybean phospholipids and caprylic acid by lipase-catalyzed acidolysis were examined in continuous packedbed enzyme reactors. Acidolysis reactions were performed in both a solvent system and a solvent-free system with the commercially immobilized lipase from Thermomyces lanuginosa (Lipozyme TL IM) as catalyst. In the packed bed reactors, different parameters for the lipase-catalyzed acidolysis were elucidated, such as solvent ratio (solvent system), temperature, substrate ratio, residence time, water content, and operation stability. The water content was observed to be very crucial for the acidolysis reaction in packed bed reactors. If no water was added to the substrate during reactions under the solvent-free system, very low incorporation corporation of caprylic acid was observed. In both solvent and solvent-free systems, acyl incorporation was favored by a high substrate ratio between acyl donor and phospholipids, a longer residence time, and a higher reaction temperature. Under certain conditions, the incorporation of around 30% caprylic acid can be obtained in continuous operation with hexane as the solvent.