Notch signalling regulates veinlet expression and establishes boundaries between veins and interveins in the Drosophila wing.
Notch signalling regulates veinlet expression and establishes boundaries between veins and interveins in the Drosophila wing.
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发表时间：
1997-05
期刊：
影响因子：
4.6
通讯作者：
J. Celis;S. Bray;A. Garcı́a-Bellido
中科院分区：
文献类型：
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作者：
J. Celis;S. Bray;A. Garcı́a-Bellido
果蝇翅膀中的静脉具有特征性的宽度，其由Notch途径的活性调节。Notch配体Delta的表达仅限于发育中的静脉，并且与Notch转录较低的地方相一致。我们发现，这种不对称的配体和受体的分布导致激活的缺口两侧的每一个静脉内的三角洲表达细胞的领土，并建立边界细胞，从相邻的interveins分离静脉。在这些细胞中，分裂基因m β的增强子的表达被激活，静脉促进基因veinlet的转录被抑制，从而限制静脉分化。我们建议，静脉厚度的建立利用包括以下机制的组合：（1）在脉间和脉区域中Notch和Delta表达的独立调节，（2）在Notch和Delta表达重叠的细胞中Delta激活Notch，（3）在Notch已被激活的细胞中Notch转录的正反馈和（4）E（spl）对细脉转录的抑制m β和通过细脉/鱼雷活性维持Delta表达。
The veins in the Drosophila wing have a characteristic width, which is regulated by the activity of the Notch pathway. The expression of the Notch-ligand Delta is restricted to the developing veins, and coincides with places where Notch transcription is lower. We find that this asymmetrical distribution of ligand and receptor leads to activation of Notch on both sides of each vein within a territory of Delta-expressing cells, and to the establishment of boundary cells that separate the vein from adjacent interveins. In these cells, the expression of the Enhancer of split gene m beta is activated and the transcription of the vein-promoting gene veinlet is repressed, thus restricting vein differentiation. We propose that the establishment of vein thickness utilises a combination of mechanisms that include: (1) independent regulation of Notch and Delta expression in intervein and vein territories, (2) Notch activation by Delta in cells where Notch and Delta expression overlaps, (3) positive feedback on Notch transcription in cells where Notch has been activated and (4) repression of veinlet transcription by E(spl)m beta and maintenance of Delta expression by veinlet/torpedo activity.