Nicking enzyme-controlled toehold regulation for DNA logic circuits
Nicking enzyme-controlled toehold regulation for DNA logic circuits
复制标题
DNA 逻辑电路的刻痕酶控制立足点调节。
DOI：
10.1039/c7nr06484e
复制
发表时间：
2017-12-14
期刊：
影响因子：
6.7
通讯作者：
Zhang, Cheng
中科院分区：
文献类型：
--
作者：
Pan, Linqiang;Wang, Zhiyu;Zhang, Cheng
DNA链置换因其显著的特异性和可预测性而被广泛应用于DNA相关的纳米工程。我们报告了一个切口酶辅助机制来调节链置换，其中DNA支点动态控制。为了证明该策略，构建了一个基于蛋白质/DNA的布尔运算系统，并在此基础上实现了一个由三种不同切口酶控制的双通道多路复用器。所提出的调节机制可用于开关逻辑语句和桥梁蛋白质和DNA逻辑电路。
DNA strand displacement is widely used in DNA-related nanoengineering for its remarkable specificity and predictability. We report a nicking enzyme-assisted mechanism to regulate strand displacement, where DNA toeholds are dynamically controlled. To demonstrate the strategy, a protein/DNA-based Boolean operation system is constructed and based on it a two-channel multiplexer controlled by three different nicking enzymes is realized. The proposed regulatory mechanism can be used for switch logic statement and bridges protein and DNA logic circuits.