Molecular definition of a novel human galectin which is immunogenic in patients with Hodgkin's disease
Molecular definition of a novel human galectin which is immunogenic in patients with Hodgkin's disease
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DOI：
10.1074/jbc.272.10.6416
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发表时间：
1997-03-07
影响因子：
4.8
通讯作者：
Sahin, U
中科院分区：
文献类型：
--
作者：
Tureci, O;Schmitt, H;Sahin, U
用自体血清免疫筛选霍奇金病组织表达文库，检测到一种新的具有Galectins(S凝集素)特征的36 kDa蛋白。对克隆的Hom-HD-21进行了序列分析，发现了两个具有半乳糖苷结合能力的同源基序，称为凝集素结构域。这两个结构域由大约30个氨基酸残基连接，序列同源性为39%。N端的凝集素结构域与已知的Galectin有中等的同源性，而C端的凝集素结构域与大鼠Galectin-5高度同源，氨基酸序列同源性为70%。我们通过与从正常外周血白细胞中克隆的相应cDNA的序列比较，排除了肿瘤来源转录本的突变。通过乳糖和半乳糖亲和层析，从裂解液中纯化了在中国仓鼠卵巢细胞中表达的重组蛋白，证明了该半乳糖凝集素的半乳糖苷结合能力。Northern印迹分析显示，表达谱仅限于外周血白细胞和淋巴组织。根据已知Galectin的命名，我们建议将这个新的半乳糖苷结合蛋白Galectin-9命名为Galectin-9。
Using autologous serum for the immunoscreening of a cDNA expression library derived from tissue involved by Hodgkin's disease, a new 36-kDa protein with the characteristics of galectins (S-type lectins) was detected. Sequence analysis of the cDNA clone HOM-HD-21 revealed two homologous motifs known as lectin domains with galactoside binding capacity. The two domains are linked by a stretch of about 30 amino acid residues and share a sequence homology of 39%. While the N-terminal lectin domain shows merely moderate homologies with known galectins, the C-terminal lectin domain is highly homologous to rat galectin-5 with an amino acid sequence identity of 70%. We ruled out mutations of the tumor-derived transcript by sequence comparison with the respective cDNA cloned from normal peripheral blood leukocytes. Recombinant protein expressed in Chinese hamster ovary cells was purified from lysates by lactose and galactose affinity chromatography, proving the galactoside binding capacity of this new galectin. Northern blot analysis revealed an expression spectrum restricted to peripheral blood leukocytes and lymphatic tissues. In accordance with the nomenclature of known galectins, we suggest to designate this novel galactoside binding protein galectin-9.