FAT1 Gene Expression in Iranian Acute Lymphoid and Myeloid Leukemia Patients.
FAT1 Gene Expression in Iranian Acute Lymphoid and Myeloid Leukemia Patients.
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DOI：
10.18502/ijhoscr.v17i2.12644
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发表时间：
2023-04-01
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背景资料：FAT非典型钙粘蛋白1（FAT 1）是钙粘蛋白超家族的成员，其丢失或获得与不同癌症的发生和/或进展相关。FAT 1过表达在血液恶性肿瘤中已有报道。本研究旨在调查伊朗成人急性白血病患者与正常动员外周血CD 34+细胞相比的FAT 1基因表达。 材料与方法：收集22例急性髓系白血病（AML）、14例急性淋巴细胞白血病（ALL）患者外周血原始细胞（外周血单个核细胞）和12例健康志愿者外周血动员的CD 34+细胞。然后，使用定量实时聚合酶链反应（qPCR）来比较FAT 1基因表达。 结果：AML和ALL患者FAT 1表达无显著性差异（p>0.2）。尽管如此，FAT 1的平均表达水平在白血病患者（AML和ALL）中显著高于正常CD 34+细胞（p=0.029）。此外，FAT 1表达水平在CD 34+和CD 34-白血病患者中均显著高于正常CD 34+细胞（p=0.028）。 结论：FAT 1在CD 34+和CD 34-白血病中的表达无显著性差异（p> 0.3）。因此，较高的FAT 1表达在ALL和AML白血病细胞中是明显的，但这似乎与CD 34表达无关。这表明在一定比例的成人急性白血病中，FAT 1表达可能被证明是治疗策略的合适靶点。
Background: FAT atypical cadherin 1 (FAT1) is a member of the cadherin superfamily whose loss or gain is associated with the initiation and/or progression of different cancers. FAT1 overexpression has been reported in hematological malignancies. This research intended to investigate FAT1 gene expression in adult Iranian acute leukemia patients, compared to normal mobilized peripheral blood CD34+ cells. Materials and Methods: The peripheral blast (peripheral blood mononuclear cells) cells of 22 acute myeloid leukemia (AML), 14 acute lymphoid leukemia (ALL) patients, and mobilized peripheral blood CD34+ cells of 12 healthy volunteer stem cell donors were collected. Then, quantitative real-time polymerase chain reaction (qPCR) was used to compare FAT1 gene expression. Results: Overall, there were no significant differences in FAT1 expression between AML and ALL patients (p>0.2). Nonetheless, the mean expression level of FAT1 was significantly higher in leukemic patients (AML and ALL) than in normal CD34+ cells (p=0.029). Additionally, the FAT1 expression levels were significantly higher in both CD34+ and CD34- leukemic patients than in normal CD34+ cells (p=0.028). Conclusion: No significant differences were found between FAT1 expression in CD34+ and CD34- leukemic samples (p> 0.3). Thus, higher FAT1 expression was evident in ALL and AML leukemia cells but this appeared unrelated to CD34 expression. This suggests in a proportion of adult acute leukemia, FAT1 expression may prove to be a suitable target for therapeutic strategies.