DNA detection using water-soluble conjugated polymers and peptide nucleic acid probes
DNA detection using water-soluble conjugated polymers and peptide nucleic acid probes
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DOI：
10.1073/pnas.162375999
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发表时间：
2002-08-20
影响因子：
11.1
通讯作者：
Bazan, GC
中科院分区：
文献类型：
--
作者：
Gaylord, BS;Heeger, AJ;Bazan, GC
利用阳离子共轭聚合物的捕光特性，对特定肽核酸（PNA）序列上的染料进行敏化发射，以实现均匀的“实时”DNA检测。信号转导是由中性PNA探针和阴性DNA靶杂交控制的。静电相互作用使杂化配合物和阳离子聚合物在福斯特能量转移所需的距离内。共轭聚合物激发提供的荧光素发射>比激发染料获得的荧光素发射>高25倍，允许用标准荧光计在10 pM浓度下检测目标DNA。一个简单和高灵敏度的分析与光学扩增，利用改进的杂交行为的PNA/DNA复合物因此证明。
The light-harvesting properties of cationic conjugated polymers are used to sensitize the emission of a dye on a specific peptide nucleic acid (PNA) sequence for the purpose of homogeneous "real-time" DNA detection. Signal transduction is controlled by hybridization of the neutral PNA probe and the negative DNA target. Electrostatic interactions bring the hybrid complex and cationic polymer within distances required for Forster energy transfer. Conjugated polymer excitation provides fluorescein emission >25 times higher than that obtained by exciting the dye, allowing detection of target DNA at concentrations of 10 pM with a standard fluorometer. A simple and highly sensitive assay with optical amplification that uses the improved hybridization behavior of PNA/DNA complexes is thus demonstrated.