GROWTH-FACTOR EFFECTS ON THE EXPRESSION OF COLLAGENASE AND TIMP-1 IN PERIODONTAL-LIGAMENT CELLS
GROWTH-FACTOR EFFECTS ON THE EXPRESSION OF COLLAGENASE AND TIMP-1 IN PERIODONTAL-LIGAMENT CELLS
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DOI：
10.1902/jop.1995.66.7.552
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发表时间：
1995-07-01
影响因子：
4.3
通讯作者：
COCHRAN, DL
中科院分区：
文献类型：
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作者：
ALVARES, O;KLEBE, R;COCHRAN, DL
成纤维细胞是牙周膜的主要细胞成分。它被认为在健康和疾病中的胶原代谢中起重要作用。牙周膜中胶原的周转被认为是由胶原合成和降解之间的平衡控制的。基质金属蛋白酶家族及其抑制剂是调节这种平衡的机制之一。牙周膜细胞合成胶原酶及其抑制剂TIMP-1的调控因素尚不清楚。本研究采用逆转录聚合酶链反应（RT-PCR）技术，观察白细胞介素-1 β（IL-1 β）、血小板源性生长因子（PDGF）和转化生长因子-β 1（TGF-β）对牙周组织成纤维细胞胶原酶（MMP-1）和金属蛋白酶组织抑制剂（TIMP-1）mRNA表达的影响。用IL-1 β（10和100 pg/ml）、PDGF的两种同种型-AA和-BB（4和20 ng/ml）和TGF-β（1和10 ng/ml）处理早期传代牙周韧带衍生的成纤维细胞。从2到24小时的生长因子处理显示，对MMP-1 mRNA的最大影响发生在24小时后。IL-1 β诱导MMP-1 mRNA增加5至9倍。PDGF的两种亚型对MMP-1 mRNA的影响较小（3至5倍），而TGF-β诱导该信息表达减少25至50%。生长因子对TIMP-1 mRNA表达无影响。这些发现表明，在所检测的生长因子中，IL-1 β在mRNA水平上对MMP-1合成产生最深远的影响。PDGF同种型的作用较小，与其他细胞类型一致，TGF-β具有抑制作用。此外，IL-1 β、PDGF和TGF-β对TIMP-1 mRNA水平没有影响。这些结果表明，在人牙周细胞，IL-1 β，PDGF-AA和-BB和TGF-β差异和特异性调节胶原酶mRNA的表达，但不是它的抑制剂。
THE FIBROBLAST IS A PROMINENT CELLULAR component of the periodontal ligament. It is believed to play an important role in collagen metabolism in health and disease. The turnover of collagen in the periodontal ligament is believed to be controlled by the balance between collagen synthesis and degradation. The family of matrix metalloproteinases and their inhibitors is one of the mechanisms which regulates this balance. The factors that regulate the synthesis of collagenase and its inhibitor, TIMP-1, by the periodontal ligament cell are poorly understood. The present study was under-taken to assess the effect of interleukin-l beta (IL-1 beta), platelet-derived growth factor (PDGF), and transforming growth factor-beta 1 (TGF-beta) on the expression of collagenase (MMP-1) and TIMP-1 mRNA in periodontal derived fibroblasts using reverse transcription polymerase chain reaction (RT-PCR). Early passage periodontal ligament derived fibroblasts were treated with IL-1 beta (10 and 100 pg/ml), two isoforms of PDGF, -AA and -BB (4 and 20 ng/ml) and TGF-beta (1 and 10 ng/ml). Treatment with growth factors from 2 to 24 hours revealed that the largest effects on MMP-1 mRNA occurred after 24 hours. IL-1 beta induced a 5 to 9 fold increase in MMP-1 mRNA. The two isoforms of PDGF had less of an effect (3 to 5 fold) on MMP-1 mRNA whereas TGF-beta induced a 25 to 50% decrease in the expression of this message. None of the growth factors had an effect on TIMP-1 mRNA expression. These findings suggest that of the growth factors examined, IL-1 beta exerts the most profound effect on MMP-1 synthesis at the mRNA level. PDGF isoforms have less of an effect and consistent with other cell types, TGF-beta had an inhibitory effect. Further, IL-1 beta, PDGF and TGF-beta had no effect on TIMP-1 mRNA level. These results demonstrate that, in human periodontal cells, IL-1 beta, PDGF-AA and -BB and TGF-beta differentially and specifically regulate expression of collagenase mRNA but not that of its inhibitor.