MOLECULAR-CLONING OF CDNA CODING FOR RAT PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA)/CYCLIN
MOLECULAR-CLONING OF CDNA CODING FOR RAT PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA)/CYCLIN
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DOI：
10.1002/j.1460-2075.1987.tb04802.x
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发表时间：
1987-03-01
期刊：
影响因子：
11.4
通讯作者：
NAKANE, PK
中科院分区：
文献类型：
--
作者：
MATSUMOTO, K;MORIUCHI, T;NAKANE, PK
增殖细胞核抗原（PCNA），也称为细胞周期蛋白，出现在细胞周期的G1/S边界。由于PCNA/cyclin与细胞增殖的可能关系，其研究受到了广泛关注。PCNA/cyclin是一种非组蛋白的酸性核蛋白，与PCNA/cyclin的表观摩尔数成正比。重量为33，000 - 36，000。兔PCNA/cyclin的氨基酸组成和前25个氨基酸的序列是已知的。用与前5个氨基酸序列相对应的寡核苷酸探针，从大鼠胸腺细胞cDNA文库中分离出PCNA/cyclin的cDNA克隆。该cDNA（1195个碱基）含有813个核苷酸的开放阅读框架，编码261个氨基酸。3“-非编码区长312个核苷酸，含有三个推定的多聚腺苷酸化信号。摩尔大鼠PCNA/细胞周期蛋白的wt计算为28，748。推导的大鼠PCNA/cyclin的氨基酸序列和组成与已发表的数据非常一致。用cDNA探针在大鼠胸腺细胞RNA中检测到两种mRNA（1.1和0.98kb）。Southern印迹分析表明，有一个单一的基因编码的PCNA/细胞周期蛋白。推导的大鼠PCNA/cyclin的氨基酸序列与单纯疱疹病毒1型DNA结合蛋白的氨基酸序列相似。
The ''proliferating cell nuclear antigen'' (PCNA), also known as cyclin, appears at the G1/S boundary in the cell cycle. Because of its possible relationship with cell proliferation, PCNA/cyclin has been receiving attention. PCNA/cyclin is a non-histone acidic nuclear protein with an apparent mol. wt of 33,000-36,000. The amino acid composition and the sequence of the first 25 amino acids of rabbits PCNA/cyclin are known. Using an oligonucletode probe corresponding to the sequence of the first five amino cids, a cDNA clone for PCNA/cyclin was isolated from rat thymocyte cDNA library. The cDNA (1195 bases) contains an open reading frame of 813 nucleotides coding for 261 amino acids. The 3''-non-coding region is 312 nucleotdies long and contains three putative polyadenylation signals. The mol. wt of rat PCNA/cyclin was calculated to be 28,748. The deduced amino acid sequence and composition of rat PCNA/cyclin are in excellent agreement with the published data. Using the cDNA probe, two species of mRNA (1.1 and 0.98 kb) were detected in rat thymocyte RNA. Southern blot analysis of total human genomic DNA suggests that there is a single gene coding for PCNA/cyclin. The deduced amino acid sequence of rat PCNA/cyclin has a similarity with that of herpes simplex virus type-1 DNA binding protein.