Recurrent papillary urothelial neoplasm of low malignant potential. Subtle architectural disorder detected by quantitative analysis in DAXX-immunostained tissue sections
Recurrent papillary urothelial neoplasm of low malignant potential. Subtle architectural disorder detected by quantitative analysis in DAXX-immunostained tissue sections
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DOI：
10.1016/j.humpath.2013.10.034
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发表时间：
2014-04-01
期刊：
影响因子：
3.3
通讯作者：
Montironi, Rodolfo
中科院分区：
文献类型：
--
作者：
Castellini, Paolo;Montironi, Maria A.;Montironi, Rodolfo
本研究的目的是鉴别低恶性潜能的非复发性和复发性乳头状尿路上皮肿瘤（PUNLMP）中所谓的结构主导序的细微变化。使用Lab VIEW （National Instruments, Austin， TX）编写的软件包对daxx免疫染色的组织切片进行定量分析。我们对12例乳头状叶沿核心纵向切开的PUNLMP进行了调查，并细分如下：7例非复发性PUNLMP和5例复发性PUNLMP。正常尿路上皮（NU） 6例。当上皮厚度阈值设置为108 μ m（即400像素）时，NU和PUNLMP之间完全分离；然而，非复发和复发病例在相同的厚度范围内。在将核延长因子阈值设定为2.1时，两个PUNLMP组、复发性PUNLMP和NU病例之间存在差异，显示出细胞核延长的比例有些相似。核取向区分非复发性和复发性PUNLMP组；然而，NU病例并没有作为两个PUNLMP组的单独一组出现。在多变量分析中结合上皮厚度，核伸长和取向，两个PUNLMP组在它们之间和NU之间似乎是分开的。NU比2个PUNLMP基团的厚度要小，并显示出伸长核和不伸长核的组合。非复发性PUNLMP的细胞核长形较多，而复发性PUNLMP的细胞核长形较少，极化程度也较低。这一发现可常规用于鉴别那些将复发的PUNLMP患者。(C) 2014爱思唯尔公司版权所有。
The aim of the study was to identify subtle changes in the so-called architectural predominant order in nonrecurrent and recurrent papillary urothelial neoplasm of low malignant potential (PUNLMP). Quantitative analysis was performed with a software package written in Lab VIEW (National Instruments, Austin, TX) in DAXX-immunostained tissue sections. Twelve cases of PUNLMP with papillary fronds sectioned lengthwise through the core were investigated and subdivided as follows: 7 nonrecurrent and 5 recurrent PUNLMP cases. Six cases of normal urothelium (NU) were included. When an epithelial thickness threshold is set at 108 mu m (ie, 400 pixels), there is a complete separation between NU and PUNLMP; however, nonrecurrent and recurrent cases fall in the same range of thickness. In setting a nuclear elongation factor threshold at 2.1, there are differences between the 2 PUNLMP groups, recurrent PUNLMP and NU cases, showing a somewhat similar proportion of elongated nuclei. The nuclear orientation separates nonrecurrent from recurrent PUNLMP groups; however, NU cases do not appear as a separate group from the 2 PUNLMP groups. In combining epithelial thickness, nuclear elongation, and orientation in a multivariate analysis, the 2 PUNLMP groups appear separate between them and from NU. NU is less thickened than the 2 PUNLMP groups and shows a combination of elongated and less elongated nuclei. Elongated nuclei are more numerous in nonrecurrent PUNLMP, whereas the nuclei in recurrent PUNLMP are less elongated and less polarized than in the other group. Such finding can be used routinely to identify those PUNLMP) patients who will have recurrence. (C) 2014 Elsevier Inc. All rights reserved.