Poly(propylene fumarate) bone tissue engineering scaffold fabrication using stereolithography: Effects of resin formulations and laser parameters
Poly(propylene fumarate) bone tissue engineering scaffold fabrication using stereolithography: Effects of resin formulations and laser parameters
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DOI：
10.1021/bm060834v
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发表时间：
2007-04-01
影响因子：
6.2
通讯作者：
Lu, Lichun
中科院分区：
文献类型：
--
作者：
Lee, Kee-Won;Wang, Shanfeng;Lu, Lichun
使用可光交联聚合生物材料的立体光刻技术是一种有效的技术，用于制造用于组织工程应用的具有受控微结构的高度复杂的三维（3D）支架。在这项研究中，我们优化了立体光刻机的紫外线固化聚合物溶液成分和激光参数。使用聚富马酸丙二醇酯（PPF）作为生物材料，使用富马酸二乙酯（DEF）作为溶剂，使用双丙烯酰氧化膦（BAPO）作为光引发剂。通过测量未交联溶液的粘度和热性能以及形成的支架的机械性能来表征三种不同重量比的 PPF/DEF 和 BAPO 含量。通过满足粘度限制和机械要求来优化树脂组合物后，通过测量不同范围的E-c和D-p在立体光刻中制造的预先设计的窗口的厚度，根据工作曲线以及激光速度和能量之间的关系确定临界曝光（E-c）和穿透深度（D-p）等激光参数。在计算机辅助设计（CAD）软件中设计具有各种孔径、孔形状和孔隙率的三维支架，并通过立体光刻技术制造。通过测量外部尺寸、孔隙率、平均孔径和压缩模量来表征制造的支架，并与 CAD 模型进行比较。实现了 xy 平面上的特征精度，并最大限度地减少了 z 轴上树脂的过度固化。具有受控微观结构的立体光刻制造的支架可用于多种组织工程应用。
Stereolithography using photo-cross-linkable polymeric biomaterials is an effective technique for fabricating highly complex three-dimensional (3D) scaffolds with controlled microstructures for tissue engineering applications. In this study, we have optimized the UV curable polymer solution composition and laser parameters for the stereolithography machine. Poly(propylene fumarate) (PPF) was used as the biomaterial, diethyl fumarate (DEF) was used as the solvent, and bisacrylphosphrine oxide (BAPO) was used as the photoinitiator. Three different weight ratios of PPF/DEF and BAPO contents were characterized by measuring the viscosities and thermal properties of the un-cross-linked solutions and the mechanical properties of the formed scaffolds. After optimizing the resin composition by satisfying both the viscosity limitation and the mechanical requirement, laser parameters such as critical exposure (E-c) and penetration depth (D-p) were determined from the working curve and the relationship between laser speed and energy by measuring the thickness of predesigned windows fabricated in stereolithography with different ranges of E-c and D-p. Three-dimensional scaffolds with various pore sizes, pore shapes, and porosities were designed in computer-aided design (CAD) software and were fabricated in stereolithography. The fabricated scaffolds were characterized by measuring external dimensions, porosities, mean pore sizes, and compressive moduli and were compared to the CAD models. Feature accuracy in the xy-plane was achieved and overcuring of the resin in z-axis was minimized. The stereolithographically fabricated scaffolds with controlled microstructures can be useful in diverse tissue engineering applications.