Molecular characterization of insulin-like peptides in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
Molecular characterization of insulin-like peptides in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
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褐飞虱 Nilaparvata lugens（半翅目：飞虱科）中胰岛素样肽的分子特征
DOI：
10.1111/imb.12636
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发表时间：
2020
影响因子：
2.6
通讯作者：
Xu H. -J.
中科院分区：
文献类型：
--
作者：
Xue W. -H.;Liu Y. -L.;Jiang Y. -Q.;He S. -F.;Wang Q. -Q.;Yang Z. -N.;Xu H. -J.
胰岛素样肽(ILPS)包括胰岛素、胰岛素样生长因子(IGF)和松弛素，是后生动物进化过程中保守的激素，参与多种生理过程。褐飞虱(BPH)编码4个ILP基因(Nlilp1、Nlilp2、Nlilp3和Nlilp4)，但其生理功能尚不清楚。序列分析表明，NlILP1含有松弛蛋白G蛋白偶联受体结合基序和半胱氨酸残基变异基序，NlILP2和NlILP4类似于脊椎动物IGFS。RNA干扰(RNAi)介导的基因沉默表明，Nlilp1、2和3的缺失显著延缓了若虫的发育历期，双基因或三基因缺失会加剧这种影响。Nlilp1、Nlilp2或Nlilp3的缺失诱导了葡萄糖、海藻糖和糖原的积累，这与BPH中胰岛素受体(NlInR1)的缺失是矛盾的。Nlilp1基因的耗尽显著提高了雌雄植株的抗饥性，但其程度小于NlInR1基因的耗竭。亲本RNAi分析表明，Nlilp1-4中的每一个基因的缺失都会极大地损害雌性的繁殖力。这些发现表明，NlILP1-4在褐飞虱的生理过程中具有多余而独特的作用，从而加深了我们对每个NlILP在自然生境中对该物种生态成功的贡献的理解。
Insulin‐like peptides (ILPs) including insulin, insulin‐like growth factor (IGF) and relaxin are evolutionarily conserved hormones in metazoans, and they are involved in diverse physiological processes. The migratory brown planthopper (BPH),Nilaparvata lugens, encodes four ILP genes (Nlilp1,Nlilp2,Nlilp3andNlilp4) but their physiological roles are largely unknown. Sequence analysis showed thatNlILP1 contained a relaxin‐specific G protein‐coupled receptor‐binding motif and a variant motif of cysteine residues, andNlILP2 andNlILP4 resembled vertebrate IGFs. RNA interference (RNAi)‐mediated gene silencing showed that depletion of each ofNlilp1,2and3significantly delayed the developmental duration of nymphs, and this effect could be exacerbated by double or triple gene depletion. Depletion ofNlilp1,Nlilp2orNlilp3induces the accumulation of glucose, trehalose and glycogen, which is contradictory to depletion of the insulin receptor (NlInR1) in the BPH. Depletion ofNlilp1significantly enhanced starvation resistance in both females and males although its extent was smaller thanNlInR1depletion. A parental RNAi assay showed that depletion of each ofNlilp1–4dramatically impaired female fecundity. These findings indicate thatNlILP1–4 have redundant and distinct roles in physiological processes in the BPH, thereby enhancing our understanding of the contribution of eachNlILP to the ecological success of this species in natural habitats.