Localization and characterization of white blood cell populations within the human ovary throughout the menstrual cycle and menopause.
Localization and characterization of white blood cell populations within the human ovary throughout the menstrual cycle and menopause.
复制标题
整个月经周期和绝经期人类卵巢内白细胞群的定位和特征。
DOI：
10.1093/oxfordjournals.humrep.a019256
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Hill,JA
中科院分区：
文献类型：
--
作者：
Best,CL;Pudney,J;Welch,WR;Burger,N;Hill,JA
这项研究的目的是定位和表征人类卵巢中的白细胞种群在其生理生命周期中的作用。对30名育龄妇女和3名绝经后妇女的卵巢进行石蜡包埋或冷冻。临床资料和病理复习被用来获得准确的月经周期信息，以确保没有卵巢疾病。组织切片进行白细胞表型染色，卵巢中的白细胞数量由两名独立的检查者使用0-3+(+)评分系统进行半定量评估。我们的结果表明，巨噬细胞和T淋巴细胞是卵巢的主要免疫细胞，它们的浓度取决于含有白细胞的结构的位置和发育阶段。发育中的卵泡在卵泡膜内含有少量(+)巨噬细胞，而闭锁卵泡的颗粒中含有中等数量的巨噬细胞(++)，而卵泡膜中含有少量(+)至中度(++)的巨噬细胞。新形成的黄体含有很少的(+)巨噬细胞，而退化的黄体含有丰富的(+++)数量。人类白细胞抗原-DR阳性细胞主要分布在巨噬细胞存在的部位，发育中的滤泡中一般不存在T淋巴细胞，但在膜血管内可见局限性的少量(+)。闭锁卵泡颗粒内T淋巴细胞很少(+)，卵泡膜内有少量(+)至中度(++)T淋巴细胞。新生的黄体中只有少量(+)T淋巴细胞，退行性黄体中有中等数量的(++)至大量的(+++)T淋巴细胞。各部位T淋巴细胞均为UCHL1阳性。黄体中T辅助细胞和CD8细胞的比例为1：1。绝经前卵巢中普遍缺乏B淋巴细胞和自然杀伤细胞。绝经后卵巢间质中仅有少量(+)巨噬细胞、T淋巴细胞和自然杀伤细胞。总之，我们的结果表明，人卵巢是一个免疫的动态组织，含有激活的巨噬细胞和T淋巴细胞，这为卵巢内的免疫内分泌相互作用提供了解剖学基础。
The purpose of this investigation was to localize and characterize white blood cell populations in the human ovary through its physiological life cycle. Ovaries from 30 women of reproductive age and from three post-menopausal women were embedded in paraffin or frozen. Clinical information and pathology review were used to obtain accurate menstrual cycle information and to ensure the absence of ovarian disease. Tissue sections were stained for leukocyte phenotypes and the numbers of white blood cells in the ovary were semiquantitatively assessed by two separate examiners using a 0–3 plus (+) scoring system. Our results demonstrated that macrophages and T lymphocytes were the primary immune cells of the ovary, the concentrations of which were dependent on the location and stage of development of the structures containing leukocytes. Developing follicles contained few (+) macrophages located in the theca, while atretic follicles possessed moderate (+ +) numbers in the granulosa and few ( + ) to moderate (+ +) numbers in the theca. Newly formed corpora lutea contained few ( + ) macrophages, while regressing corpora lutea contained abundant (+ + +) numbers. Human leukocyte antigen (HLA)-DR positive cells were located predominantly at sites where macrophages were present T lymphocytes were generally not present in the developing follicle but focal, small (+) numbers were observed in blood vessels of the theca. Atretic follicles contained few ( + ) T lymphocytes in the granulosa and few (+) to moderate ( + +) numbers in the theca. Few (+ ) T lymphocytes were present in new corpora lutea, while moderate (+ +) to abundant (+ + +) numbers were present in regressing corpora lutea. T lymphocytes at all sites were UCHLl positive. The CD4 (T helper) to CD8 (T suppressor) ratio in the corpus luteum was 1: 1. B-lymphocytes and natural killer cells were generally absent in the pre-menopausal ovary. Hie post-menopausal ovary, in contrast, only contained few ( + ) macrophages, T lymphocytes and natural killer cells in the stroma. In conclusion, our results indicate that the human ovary is an immunologicaUy dynamic tissue containing activated macrophages and T lymphocytes which provide an anatomical basis for immunoendocrine interactions within the ovary.