Size and UV germicidal irradiation susceptibility of Serratia marcescens when aerosolized from different suspending media
Size and UV germicidal irradiation susceptibility of Serratia marcescens when aerosolized from different suspending media
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DOI：
10.1128/aem.70.4.2021-2027.2004
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发表时间：
2004-04-01
影响因子：
4.4
通讯作者：
First, MW
中科院分区：
文献类型：
--
作者：
Lai, KM;Burge, HA;First, MW
世界各地的实验室都建立了实验系统，以研究各种环境参数对紫外线杀菌照射 (UVGI) 灭活空气微生物的功效的影响。人们普遍认为，由于系统和实验条件的差异，来自不同实验室的数据可能会有很大差异。在这项研究中，我们在实验室建立的实验系统中研究了悬浮介质的成分对粘质沙雷氏菌大小和 UVGI 敏感性的影响。将粘质沙门氏菌悬浮在（i）蒸馏水，（ii）磷酸盐缓冲液，（iii）10％胎牛血清，（iv）磷酸盐缓冲盐水（盐水，0.8％氯化钠）和（v）合成唾液（含10％胎牛血清的磷酸盐缓冲盐水）中。在低湿度（36%）下，悬浮在纯水介质中的粘质链球菌最容易受到 UVGI 的影响，其次是纯血清介质中的粘质链球菌。来自纯水和纯血清培养基的可培养颗粒的计数中值直径 (CMD) 分别为 0.88 和 0.95 μm，测量结果基于其空气动力学行为。悬浮在磷酸盐缓冲液、合成唾液和磷酸盐缓冲盐水中的细菌具有相似的UVGI敏感性和CMD，分别为1.0、1.4和1.5μm。在高湿度（68％）下，颗粒的CMD增加了6％至16％，同时UVGI敏感性下降，下降幅度与悬浮介质的类型有关。总之，悬浮介质的选择影响粘质链球菌的大小和 UVGI 敏感性。这些数据对于进行比较和决定针对各种应用使用适当的介质非常有价值。
Experimental systems have been built in laboratories worldwide to investigate the influence of various environmental parameters on the efficacy of UV germicidal irradiation (UVGI) for deactivating airborne microorganisms. It is generally recognized that data from different laboratories might vary significantly due to differences in systems and experimental conditions. In this study we looked at the effect of the composition of the suspending medium on the size and UVGI susceptibility of Serratia marcescens in an experimental system built in our laboratory. S. marcescens was suspended in (i) distilled water, (ii) phosphate buffer, (iii) 10% fetal calf serum, (iv) phosphate-buffered saline (saline, 0.8% sodium chloride), and (v) synthetic saliva (phosphate-buffered saline with 10% fetal calf serum). At low humidity (36%), S. marcescens suspended in water-only medium was the most susceptible to UVGI, followed by those in serum-only medium. The count median diameters (CMDs) for culturable particles from water-only and serum-only media were 0.88 and 0.95 mum, respectively, with the measurements based on their aerodynamic behavior. The bacteria suspended in phosphate buffer, synthetic saliva, and phosphate-buffered saline had similar UVGI susceptibility and CMD at 1.0, 1.4, and 1.5 mum, respectively. At high humidity (68%) the CMD of the particles increased by 6 to 16%, and at the same time UVGI susceptibility decreased, with the magnitude of decrease related to the type of suspending medium. In conclusion, the choice of suspending medium influenced both size and UVGI susceptibility of S. marcescens. These data are valuable for making comparisons and deciding on the use of an appropriate medium for various applications.