A rapid method combining Golgi and Nissl staining to study neuronal morphology and cytoarchitecture
A rapid method combining Golgi and Nissl staining to study neuronal morphology and cytoarchitecture
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DOI：
10.1369/jhc.2008.950246
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发表时间：
2008-06-01
影响因子：
3.2
通讯作者：
Hamann, Martine
中科院分区：
文献类型：
--
作者：
Pilati, Nadia;Barker, Matthew;Hamann, Martine
高尔基体银浸渍技术给出了它标记的少数神经元的神经元形态的详细信息，而大多数神经元保持未染色。相比之下，尼氏染色技术允许整个神经元群体的一致标记，但给出了非常有限的信息神经元形态。大多数表征神经元细胞类型在其在组织切片内的分布的背景下的研究倾向于使用高尔基体银浸渍技术用于神经元形态学，然后脱浸渍作为通过随后的尼氏染色显示神经元的组织学位置的先决条件。在这里，我们描述了一种快速的方法结合高尔基体银浸渍与甲酚紫染色，提供了一个有用的和简单的方法相结合的细胞形态与细胞结构，而不需要脱浸渍的组织。我们的方法使我们能够识别面神经核和三叉神经上核的神经元，以及评估耳蜗背核层内的细胞分布。用这种方法，我们也已经能够直接比较形态特征的神经元胞体在耳蜗背核标记时，与高尔基体的方法得到的甲酚紫，我们发现，甲酚紫标记的细胞体出现在高细胞密度较小。我们的观察表明，甲酚紫染色是不足以量化的差异，索马大小。
The Golgi silver impregnation technique gives detailed information on neuronal morphology of the few neurons it labels, whereas the majority remain unstained. In contrast, the Nissl staining technique allows for consistent labeling of the whole neuronal population but gives very limited information on neuronal morphology. Most studies characterizing neuronal cell types in the context of their distribution within the tissue slice tend to use the Golgi silver impregnation technique for neuronal morphology followed by deimpregnation as a prerequisite for showing that neuron's histological location by subsequent Nissl staining. Here, we describe a rapid method combining Golgi silver impregnation with cresyl violet staining that provides a useful and simple approach to combining cellular morphology with cytoarchitecture without the need for deimpregnating the tissue. Our method allowed us to identify neurons of the facial nucleus and the supratrigeminal nucleus, as well as assessing cellular distribution within layers of the dorsal cochlear nucleus. With this method, we also have been able to directly compare morphological characteristics of neuronal somata at the dorsal cochlear nucleus when labeled with cresyl violet with those obtained with the Golgi method, and we found that cresyl violet-labeled cell bodies appear smaller at high cellular densities. Our observation suggests that cresyl violet staining is inadequate to quantify differences in soma sizes.