Nucleotide and deduced amino acid sequence of the alpha subunit of yeast pyruvate dehydrogenase.
Nucleotide and deduced amino acid sequence of the alpha subunit of yeast pyruvate dehydrogenase.
复制标题
酵母丙酮酸脱氢酶α亚基的核苷酸和推导的氨基酸序列。
DOI：
10.1016/0006-291x(89)91549-0
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Reed,LJ
中科院分区：
文献类型：
--
作者：
Behal,RH;Browning,KS;Reed,LJ
A 413-base cDNA insert encoding a portion of the alpha subunit of pyruvate dehydrogenase (E 1 α; EC 1.2. 4.1) from Saccharomyces cerevisiae was isolated from a λgtll cDNA library by immunoscreening and by hybridization with an oligonucleotide probe which corresponded to the amino acid sequence around the phosphorylation site of E 1 α. This cDNA was subcloned, sequenced and used as a probe to isolate two additional cDNA inserts which were subcloned and sequenced. These overlapping clones comprised the carboxyl-terminal part of E 1 α. To identify the missing nucleotide sequence, the polymerase chain reaction was used to amplify yeast genomic DNA with synthetic oligonucleotide primers based on the amino-terminal sequence of E 1 α and the 5′ end of one of the cDNA clones. Three DNA fragments were isolated and sequenced. The composite nucleotide sequence has an open reading frame of 1260 nucleotides encoding a putative presequence of 33 amino acids and a mature protein of 387 amino acids (M r= 42,703). Hybridization analysis showed that the size of the mRNA is about I. 4 kilobases.
