TYPES AND SPATIAL-DISTRIBUTION OF VASOACTIVE INTESTINAL POLYPEPTIDE (VIP) CONTAINING SYNAPSES IN THE RAT VISUAL-CORTEX
TYPES AND SPATIAL-DISTRIBUTION OF VASOACTIVE INTESTINAL POLYPEPTIDE (VIP) CONTAINING SYNAPSES IN THE RAT VISUAL-CORTEX
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DOI：
10.1007/bf00318224
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发表时间：
1988-01-01
期刊：
影响因子：
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通讯作者：
KALMAN, M
中科院分区：
文献类型：
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作者：
HAJOS, F;ZILLES, K;KALMAN, M
用光镜、电子显微镜和图像分析方法研究了大鼠视皮质血管活性肠肽(VIP)的结构。VIP免疫反应轴突终末与锥体细胞和中间神经元的树突、树突、胞体形成对称性突触。VIP终末经常与VIP阴性的不对称突触在同一突触后结构上成对出现。VIP免疫染色的树突和核周分别由VIP阴性的纯不对称终末和混合终末联系。与其他类型的VIP突触相比，两个VIP神经元之间的突触联系很少。通过对光镜制剂中VIP染色的颗粒和树枝状颗粒的图像分析获得的定量研究表明，其具有明显的层状分布。树枝状颗粒在第I-II层最常见，而轴突有三层堆积：在第I-II层的边界，在第IV层和第VI层。结合前人的结果，本研究结果表明VIP-BOTON在空间上是非随机分布的。
In the rat visual cortex vasoactive intestinal polypeptide (VIP)-containing structures were studied by means of light and electron microscopy and image analysis. VIP-immunoreactive axon terminals were found to form symmetric synapses with small dendritic shafts, dendritic spines and somata of pyramidal cells and interneurons. VIP-terminals often occured in pairs with VIP-negative, asymmetric synapses on the same postsynaptic structure. VIP-immunostained dendrites and perikarya were contacted by a purely asymmetric and a mixed population of VIP-negative terminals, respectively. Synaptic connections between two VIP-neurons are seldom as compared to the other types of VIP-synapses. Quantitative studies obtained by the image analysis of VIP-stained boutons and dendritic particles in light microscopic preparations suggest a distinct laminar distribution. Dendritic particles are most frequent in layers I–II, whereas axonal boutons have three laminar accumulations: at the border of layers I–II, in layer IV and layer VI. Together with previous results, the present findings argue for a non-random spatial distribution of VIP-boutons.