CYTOSKELETAL STRUCTURE WITH ASSOCIATED POLYRIBOSOMES OBTAINED FROM HELA-CELLS
CYTOSKELETAL STRUCTURE WITH ASSOCIATED POLYRIBOSOMES OBTAINED FROM HELA-CELLS
复制标题
DOI：
10.1016/0092-8674(77)90141-6
复制
发表时间：
1977-01-01
期刊：
影响因子：
64.5
通讯作者：
PENMAN, S
中科院分区：
文献类型：
--
作者：
LENK, R;RANSOM, L;PENMAN, S
本文描述了一种方法，通过该方法可以对海拉（人宫颈癌）细胞进行分级分离，从而在细胞质中显示出一种类似骨架的结构。这种细胞骨架具有细胞的许多超微结构特征，例如100埃的细丝、微丝、中心粒和微棘，尽管大部分细胞蛋白质、细胞膜和微管已被提取出来。与细胞骨架相关联的是大多数多聚核糖体，但不是单体核糖体。这些多聚核糖体分布在整个细胞骨架中，但100埃细丝所在区域除外，这与完整细胞中的分布情况相似。用低水平的核糖核酸酶降解信使核糖核酸会使大多数核糖体从细胞骨架上释放出来。体内多聚核糖体预先解聚会释放核糖体但不会释放信使核糖核酸。体内施用细胞松弛素B会使信使核糖核酸从细胞骨架上释放出来。这些结果表明多聚核糖体通过信使核糖核酸附着在细胞骨架上。
A method is described by which HeLa [human cervical carcinoma] cells can be fractionated to reveal a skeletal-like structure in the cytoplasm. This cytoskeleton has many of the cell''s ultrastructural features, such as 100.ANG. filaments, microfilaments, centrioles and microspikes, although most of the cellular protein, membranes, and microtubules were extracted. Associated with the cytoskeleton are most of the polysomal, but not the monomeric, ribosomes. These polysomes are distributed throughout the cytoskeleton except in the region of the 100.ANG. filaments, which resembles the distribution in intact cells. Degradation of mRNA with low levels of RN releases most ribosomes from the cytoskeleton. Prior disaggregation of polyribosomes in vivo releases ribosomes but not mRNA. Cytochalasin B administered in vivo releases the mRNA from the cytoskeleton. These results suggest an attachment of polyribosomes to the cytoskeleton via mRNA.