A COMPARATIVE-STUDY OF HISTOLOGICAL AND IMMUNOHISTOCHEMICAL METHODS FOR NEUROFIBRILLARY TANGLES AND SENILE PLAQUES IN ALZHEIMERS-DISEASE
A COMPARATIVE-STUDY OF HISTOLOGICAL AND IMMUNOHISTOCHEMICAL METHODS FOR NEUROFIBRILLARY TANGLES AND SENILE PLAQUES IN ALZHEIMERS-DISEASE
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DOI：
10.1007/bf00308476
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发表时间：
1992-01-01
影响因子：
12.7
通讯作者：
BOURAS, C
中科院分区：
文献类型：
--
作者：
VALLET, PG;GUNTERN, R;BOURAS, C
一些研究已经证明，神经退行性疾病中病理病变的准确可视化和定量取决于染色方法的可靠性。为了更好地评估阿尔茨海默病神经病理学标志物的密度和分布，我们比较了改良的硫代黄素S方案对神经纤维缠结（NFT）和老年斑（SP）的染色效率与不同的银浸渍技术（Bielchowsky，Gallyas，Globus，Campbell-Switzer-Martin）和用针对淀粉样β蛋白A4和微管相关tau蛋白的两种不同抗体获得的免疫组织化学染色。与Bielschowsky和Globus方法相比，改良的硫代黄素S技术（MTST）分别检测到多60%的SP和多50%的NFT。用特异性抗体获得的结果与用MTST获得的结果相当，但是这些免疫技术对于常规神经病理学评价来说更昂贵和耗时，并且适当的抗体并不总是可商购的。此外，与经典的硫磺素S相比，NFT和SP与MTST的形态外观大大改善，并且特异性染色的结构和背景之间的信噪比增加，允许准确的半自动定量。
Several studies have demonstrated that the accurate visualization and quantification of pathological lesions in neurodegenerative disorders depend on the reliability of staining methods. In an attempt to gain a better assessment of the density and distribution of the neuropathological markers of Alzheimer's disease, we compared the staining efficiency of a modified thioflavine S protocol for neurofibrillary tangles (NFT) and senile plaques (SP) to different argentic impregnation techniques (Bielchowsky, Gallyas, Globus, Campbell-Switzer-Martin) and to immunohistochemical stainings obtained with two different antibodies against the amyloid beta-protein A4 and the microtubule-associated tau protein. The modified thioflavine S technique (MTST) detects up to 60 % more SP and up to 50 % more NFT than the Bielschowsky and Globus methods, respectively. The results obtained with the specific antibodies are comparable to those obtained with the MTST, but these immunotechniques are more expensive and time consuming for routine neuropathological evaluation, and the appropriate antibodies are not always commercially available. Furthermore, the morphological appearance of NFT and SP with MTST is greatly improved when compared to the classical thioflavine S and the increased signal-to-noise ratio between specifically stained structures and background permits an accurate semi-automatic quantification.