An Arabidopsis cell wall-associated kinase required for invertase activity and cell growth
An Arabidopsis cell wall-associated kinase required for invertase activity and cell growth
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DOI：
10.1111/j.1365-313x.2006.02695.x
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发表时间：
2006-04-01
期刊：
影响因子：
7.2
通讯作者：
Byers, N
中科院分区：
文献类型：
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作者：
Kohorn, BD;Kobayashi, M;Byers, N
壁相关蛋白(WAK)是一个由5个蛋白质组成的家族，它含有胞外结构域，可以与细胞壁的果胶分子相连，它跨越质膜，具有细胞质丝氨酸/苏氨酸激酶结构域。以前的工作表明，WAK蛋白水平的降低会导致细胞扩张的丧失，这表明这些受体样蛋白在细胞形状形成中发挥了作用。在这里，它表明了单一的wak2突变表现出对糖和盐对幼苗生长的依赖性。这种突变还会降低液泡转化酶的表达和活性，这通常是导致肿胀和扩张的关键因素。因此，WAKS可以提供一种分子机制，将细胞壁感觉(通过果胶附着)与溶质代谢的调节联系起来，这反过来又被认为与生长细胞的膨胀性维持有关。
The wall-associated kinases (WAK), a family of five proteins that contain extracellular domains that can be linked to pectin molecules of the cell wall, span the plasma membrane and have a cytoplasmic serine/threonine kinase domain. Previous work has shown that a reduction in WAK protein levels leads to a loss of cell expansion, indicating that these receptor-like proteins have a role in cell shape formation. Here it is shown that a single wak2 mutation exhibits a dependence on sugars and salts for seedling growth. This mutation also reduces the expression and activity of vacuolar invertase, often a key factor in turgor and expansion. WAKs may thus provide a molecular mechanism linking cell wall sensing (via pectin attachment) to regulation of solute metabolism, which in turn is known to be involved in turgor maintenance in growing cells.