GROWTH CONE NEUROTRANSMITTER RECEPTOR ACTIVATION MODULATES ELECTRIC FIELD-GUIDED NERVE GROWTH
GROWTH CONE NEUROTRANSMITTER RECEPTOR ACTIVATION MODULATES ELECTRIC FIELD-GUIDED NERVE GROWTH
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DOI：
10.1006/dbio.1995.1285
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发表时间：
1995-10-01
影响因子：
2.7
通讯作者：
MCCAIG, CD
中科院分区：
文献类型：
--
作者：
ERSKINE, L;MCCAIG, CD
我们已经研究了两个神经引导线索之间的相互作用，单独诱导大量的生长锥转向：内源性神经递质和小DE电场。d-筒箭毒碱，烟碱AChR（乙酰胆碱受体）拮抗剂，抑制held诱导的阴极方向培养的神经突，而阿托品，毒蕈碱AChR阻断剂，和苏拉明，P-2-嘌呤受体拮抗剂，显着增强的应用领域的指导性能。这些实验牵连的生长锥烟碱乙酰胆碱受体的自释放的乙酰胆碱在电场诱导的神经突方向的基础机制的激活，并提出了可能性，生长锥释放神经递质之前的目标相互作用，以协助自己的寻路。此外，他们提供了第一个证据表明，几种神经递质受体的共激活可能相互作用，以调节定向神经生长。这种相互作用在体内，其中指导信号共存，将增加进一步的控制水平，以神经突的指导。(C)出版社：Academic Press
We have studied the interactions between two nerve guidance cues, which alone induce substantial growth cone turning: endogenous neurotransmitters and small de electric fields. d-tubocurarine, a nicotinic AChR (acetylcholine receptor) antagonist, inhibited held-induced cathodal orientation of cultured neurites, whereas atropine, a muscarinic AChR blocker, and suramin, a P-2-purinoceptor antagonist, markedly enhanced the guidance properties of the applied field. These experiments implicate the activation of growth cone nicotinic AChRs by self-released acetylcholine in the mechanism underpinning electric field-induced neurite orientation and raise the possibility that growth cones release neurotransmitter prior to target interaction in order to assist their own pathfinding. Additionally, they provide the first evidence that coactivation of several neurotransmitter receptors may interact to regulate directed nerve growth. Such interaction in vivo, where guidance signals coexist, would add further levels of control to neurite guidance. (C) 1995 Academic Press, Inc.