Fiber-based 1150-nm femtosecond laser source for the minimally invasive harmonic generation microscopy
Fiber-based 1150-nm femtosecond laser source for the minimally invasive harmonic generation microscopy
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DOI：
10.1117/1.jbo.22.3.036008
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发表时间：
2017-03-01
影响因子：
3.5
通讯作者：
Liu, Tzu-Ming
中科院分区：
文献类型：
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作者：
Huang, Jing-Yu;Guo, Lun-Zhang;Liu, Tzu-Ming
谐波发生显微镜（HGM）已成为一种独特的光学虚拟活检工具，用于癌症诊断和体内白细胞计数。无需标记，HGM可以揭示体内组织和细胞的亚微米特征。为了获得更深的成像深度和最小的侵入性，人们通常采用1100至1300 nm的飞秒激光源。然而，这些激光器通常基于大体积的振荡器，其性能对环境条件敏感。我们展示了一个基于光纤的1150 nm飞秒激光源，具有6.5 nJ的脉冲能量，86 fs的脉冲宽度，和11.25 MHz的脉冲重复率。它是由硼酸铋或镁掺杂的周期性极化的锂的硼酸盐（MgO：PPLN）介导的2300 nm的孤子，从1550 nm的飞秒脉冲的激发大模面积的光子晶体光纤产生的倍频。结合自制的激光扫描显微镜和定制的图像采集卡，我们实现了30 Hz帧速率下的每像素脉冲HGM体内成像。这种集成解决方案有可能被开发为用于常规临床使用的稳定HGM系统。(C)2017年，摄影光学仪器工程师协会（SPIE）
Harmonic generation microscopy (HGM) has become one unique tool of optical virtual biopsy for the diagnosis of cancer and the in vivo cytometry of leukocytes. Without labeling, HGM can reveal the submicron features of tissues and cells in vivo. For deep imaging depth and minimal invasiveness, people commonly adopt 1100- to 1300-nm femtosecond laser sources. However, those lasers are typically based on bulky oscillators whose performances are sensitive to environmental conditions. We demonstrate a fiber-based 1150-nm femtosecond laser source, with 6.5-nJ pulse energy, 86-fs pulse width, and 11.25-MHz pulse repetition rate. It was obtained by a bismuth borate or magnesium-doped periodically poled lithium niobate (MgO:PPLN) mediated frequency doubling of the 2300-nm solitons, generated from an excitation of 1550-nm femtosecond pulses on a large mode area photonic crystal fiber. Combined with a home-built laser scanned microscope and a tailor-made frame grabber, we achieve a pulse-per-pixel HGM imaging in vivo at a 30-Hz frame rate. This integrated solution has the potential to be developed as a stable HGM system for routine clinical use. (C) 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)