A MUTANT OF HERPES-SIMPLEX VIRUS TYPE-1 IN WHICH THE UL13 PROTEIN-KINASE GENE IS DISRUPTED
A MUTANT OF HERPES-SIMPLEX VIRUS TYPE-1 IN WHICH THE UL13 PROTEIN-KINASE GENE IS DISRUPTED
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DOI：
10.1099/0022-1317-74-3-387
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发表时间：
1993-03-01
影响因子：
3.8
通讯作者：
MCGEOCH, DJ
中科院分区：
文献类型：
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作者：
COULTER, LJ;MOSS, HWM;MCGEOCH, DJ
单纯疱疹病毒1型（HSV-1）的基因UL 13先前已被提出编码蛋白激酶。通过插入大肠杆菌lacZ基因，构建了具有失活的UL 13的HSV-1突变体。发现该UL 13-lacZ突变体在组织培养中生长至接近野生型（wt）滴度。wt和UL 13-lacZ病毒体的银染SDS-PAGE图谱的比较表明，UL 13蛋白是wt病毒体的容易检测的组分，位于被膜中，并且可能等同于先前描述的物种VP 18。8.用病毒感染的细胞的核提取物和用去污剂处理的病毒体进行的体外磷酸化研究表明，UL 13蛋白参与被膜蛋白VP 22的磷酸化。工程化以表达UL 13并用UL 13-lacZ病毒感染的细胞的提取物也能够进行VP 22磷酸化。
Gene UL13 of herpes simplex virus type 1 (HSV-1) has previously been proposed to encode a protein kinase. An HSV-1 mutant with UL13 inactivated by insertion of the Escherichia coli lacZ gene was constructed. This UL 13-lacZ mutant was found to grow to near wild-type (wt) titres in tissue culture. Comparison of silver-stained SDS-PAGE profiles of wt and UL13-lacZ virions demonstrated that the UL13 protein is a readily detectable component of wt virions, located in the tegument and probably equivalent to the previously described species VP 1 8. 8. Studies of in vitro phosphorylation with nuclear extracts of virus-infected cells and with detergent-treated virions showed that the UL13 protein is involved in phosphorylation of the tegument protein VP22. Extracts of cells engineered to express UL13, and infected with UL13-lacZ virus, were also capable of VP22 phosphorylation.