Follicular dendritic cells are related to bone marrow stromal cell progenitors and to myofibroblasts
Follicular dendritic cells are related to bone marrow stromal cell progenitors and to myofibroblasts
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DOI：
10.4049/jimmunol.177.1.280
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发表时间：
2006-07-01
影响因子：
4.4
通讯作者：
Olivares, Enrique G.
中科院分区：
文献类型：
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作者：
Munoz-Fernandez, Raquel;Blanco, Francisco Javier;Olivares, Enrique G.
滤泡树突状细胞（FDC）参与在二次免疫应答期间将天然Ag呈递给B细胞。一些作者认为FDC是造血细胞，而另一些作者认为它们是间充质细胞。FDC在淋巴滤泡中的比例低，再加上分离技术上的困难，使得这些细胞难以研究。我们表明成纤维细胞培养基可以成功地用于分离和维持FDC细胞系。在此培养基中，我们从人扁桃体中获得了18个FDC细胞系，其增殖长达18周，并通过流式细胞术和RT-PCR检测显示出稳定的Ag表型。FDC细胞系是CD 45阴性的，表达与FDC相关的Ag（CD 21、CD 23、CD 35、CD 40、CD 73、BAFF、ICAM-1和VCAM-1）和特异于。FDC（DRC-1、CNA.42和HJ 2）。这些细胞系还能够结合B细胞并分泌CXCL 13，这是FDC的功能活性特征。然而，STRO-1以及CD 10、CD 13、CD 29、CD 34、CD 63、CD 73、CD 90、ICAM-1、VCAM-1、HLA-DR、碱性磷酸酶和α-平滑肌肌动蛋白（α-SM肌动蛋白）的额外表达表明FDC与骨髓基质细胞祖细胞密切相关。α-SM肌动蛋白的表达也使FDC与肌成纤维细胞相关。与肌成纤维细胞一样，FDC细胞系表达含有α-SM肌动蛋白的应力纤维，并且能够在TGF β 1和血小板衍生生长因子的作用下收缩胶原凝胶。这些发现表明FDC是肌成纤维细胞的一种特化形式，来源于骨髓基质细胞祖细胞。
Follicular dendritic cells (FDC) are involved in the presentation of native Ags to B cells during the secondary immune response. Some authors consider FDC to be hemopoietic cells, whereas others believe them to be mesenchymal cells. The low proportion of FDC in the lymphoid follicle, together with technical difficulties in their isolation, make these cells difficult to study. We show that Fibroblast Medium can be used successfully to isolate and maintain FDC lines. In this culture medium, we obtained 18 FDC lines from human tonsils, which proliferated for as long as 18 wk and showed a stable Ag phenotype as detected by flow cytometry and RT-PCR. FDC lines were CD45-negative and expressed Ags associated to FDC (CD21, CD23, CD35, CD40, CD73, BAFF, ICAM-1, and VCAM-1) and Ags specific for. FDC (DRC-1, CNA.42, and HJ2). These cell lines were also able to bind B cells and secrete CXCL13, functional activities characteristic of FDC. Nevertheless, the additional expression of STRO-1, together with CD10, CD13, CD29, CD34, CD63, CD73, CD90, ICAM-1, VCAM-1, HLA-DR, alkaline phosphatase, and a-smooth muscle actin (alpha-SM actin) indicated that FDC are closely related to bone marrow stromal cell progenitors. The expression of a-SM actin also relates FDC with myofibroblasts. Like myofibroblasts, FDC lines expressed stress fibers containing a-SM actin and were able to contract collagen gels under the effect of TGF beta 1 and platelet-derived growth factor. These findings suggest that FDC are a specialized form of myofibroblast and derive from bone marrow stromal cell progenitors.