High-Throughput Imaging of Bacillus subtilis.
High-Throughput Imaging of Bacillus subtilis.
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DOI：
10.1007/978-1-0716-2221-6_19
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发表时间：
2022
影响因子：
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通讯作者：
Paula Montero Llopis;Ryan Stephansky;Xindan Wang
中科院分区：
文献类型：
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作者：
Paula Montero Llopis;Ryan Stephansky;Xindan Wang
枯草芽孢杆菌是一种广泛用于研究细胞过程和发育的模式菌.阵列突变体库的可用性使我们有机会对每个突变体进行细胞学分析，并筛选参与细胞形状决定、细胞分裂和染色体分离的新基因。在这里，我们描述了一种高通量的方法来成像arrayedB。使用宽视野荧光显微镜对枯草杆菌突变体文库进行鉴定。我们提供了一个详细的描述增长的阵列应变收集，准备载玻片含有琼脂糖凝胶，设置显微镜程序，获取图像，并分析图像。
Bacillus subtilisis a widely used model bacterium to study cellular processes and development. The availability of an arrayed mutant library gave us the opportunity to cytologically analyze every mutant and screen for new genes involved in cell shape determination, cell division, and chromosome segregation. Here we describe a high-throughput method to image arrayedB. subtilismutant libraries using wide-field fluorescence microscopy. We provide a detailed description of growing the arrayed strain collection, preparing slides containing agarose pedestals, setting up the microscopy procedure, acquiring images, and analyzing the images.