achaete, but not scute, is dispensable for the peripheral nervous system of Drosophila
achaete, but not scute, is dispensable for the peripheral nervous system of Drosophila
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DOI：
10.1016/j.ydbio.2005.06.025
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发表时间：
2005-09-15
影响因子：
2.7
通讯作者：
Simpson, P
中科院分区：
文献类型：
--
作者：
Marcellini, S;Gibert, JM;Simpson, P
果蝇的无壳盾复合体一直是广泛遗传和发育分析的焦点。在该位点的四个基因中，achaete 和 scute 似乎在指定周围神经系统方面发挥着多余的作用。它们共享顺式调控元件并在相同位置共同表达。之前已经描述了去除盾片活性的突变；它会导致一些感觉鬃毛的损失。因此，当 Scute 不存在时，Achaete 的活性允许形成剩余的刷毛。然而，所有现有的无毛藻突变体都是影响无毛藻和盾片共有的调节序列的重排。为了确定两个基因之间的冗余水平，我们使用 P 元件方法来生成 achaete 的无效等位基因，从而使盾片和所有顺式调控元件保持完整。我们发现无毛虫突变幼虫和成虫的周围神经系统缺乏任何可检测的表型。然而，当盾甲的水平受到限制时，无毛果突变果蝇就会缺失一些感觉器官。 achaete 和 scute 被认为是由大约 100 密尔前的一次复制事件产生的。无毛蝇和盾蝇之间的差异令人惊讶，并提出了在进化过程中保留这两种基因的问题。 (c) 2005 Elsevier Inc. 保留所有权利。
The achaete-scute complex of Drosophila has been the focus of extensive genetic and developmental analysis. Of the four genes at this locus, achaete and scute appear to act redundantly to specify the peripheral nervous system. They share cis-regulatory elements and are co-expressed at the same locations. A mutation removing scute activity has been previously described; it causes a loss of some sensory bristles. Thus, when Scute is absent, the activity of achaete allows formation of the remaining bristles. However, all existing achaete mutants are rearrangements affecting regulatory sequences common to both achaete and scute. To determine the level of redundancy between the two genes, we have used a P element approach to generate a null allele of achaete, which leaves scute and all cis-regulatory elements intact. We find that the peripheral nervous system of achaete null mutant larvae and imagos lacks any detectable phenotype. However, when the levels of Scute are limiting, then some sensory organs are missing in achaete mutant flies. achaete and scute are thought to have arisen from a duplication event about 100 Myr ago. The difference between achaete and scute null flies is surprising and raises the question of the retention of both genes during the course of evolution. (c) 2005 Elsevier Inc. All rights reserved.