Hormone-specific suppression of adenosine 3',5'-monophosphate responses in bone in vitro during prolonged incubation with parathyroid hormone, prostaglandin E1, and calcitonin.
Hormone-specific suppression of adenosine 3',5'-monophosphate responses in bone in vitro during prolonged incubation with parathyroid hormone, prostaglandin E1, and calcitonin.
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在与甲状旁腺激素、前列腺素 E1 和降钙素长时间孵育期间，激素特异性抑制体外骨中腺苷 3,5-单磷酸反应。
DOI：
10.1210/endo-103-2-333
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发表时间：
1978
期刊：
影响因子：
4.8
通讯作者：
B. Ng
中科院分区：
文献类型：
--
作者：
J. Heersche;M. Heyboer;B. Ng
孵育胎鼠颅盖与甲状旁腺激素（PTH，2 U/ml）导致cAMP浓度在组织中的初始上升，在15分钟后，由进一步孵育后减少，但浓度保持在控制水平以上至少120分钟。cAMP水平的下降不能解释激素失活或分泌到培养基中的激素抑制剂。用PTH孵育60分钟的颅骨对添加新的PTH没有反应，但此时添加鲑鱼降钙素（sCT，100 mU/ml）或前列腺素E1（PGE 1，2.5 μg/ml）明显导致cAMP浓度进一步增加。长时间孵育的颅盖骨与sCT，PGE 1，和PGE 2也引起了显着增加的cAMP浓度在15分钟，随后下降。与sCT孵育60分钟的组织对添加新的sCT没有反应，但对新添加的PTH有反应。同样，与PGE 1孵育60分钟的组织对加入新的PGE 1没有反应，但对加入新的PGE 1有反应。
Incubation of fetal rat calvaria with parathyroid hormone (PTH, 2 U/ml) causes an initial rise in cAMP concentration in the tissue at 15 min, followed by a decrease upon further incubation, but concentrations remain above control levels for at least 120 min. The decrease in cAMP levels could not be explained by hormone inactivation or by secretion into the medium of a hormone inhibitor. Calvaria incubated for 60 min with PTH did not respond to the addition of new PTH, but addition of salmon calcitonin (sCT, 100 mU/ml) or prostaglandin E1 (PGE1, 2.5 μg/ml) at this point clearly resulted in a further increase in cAMP concentration. Prolonged incubation of calvaria with sCT, PGE1, and PGE2 also caused a significant increase in concentration of cAMP at 15 min, followed by a decrease thereafter. Tissue incubated with sCT for 60 min did not respond to the addition of new sCT, but responded to newly added PTH. Similarly, tissue incubated for 60 min with PGE1, did not respond to addition of new PGE1, but responde...